Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241030163330_jv9z ATACTGTGGAGGGCTGGCCGGGCAGTCGGG(+TTATGTAACAT)TGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030163221_shfd ATACTGTGGAGGGCTGGCC(+TTATGTAACAT)GGGCAGTCGGGTGCAAGGAAGAAGCCAGAAACCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGC NGG 10 8 17 8 24 0 100 5 10
    241030144401_hnfr taggcgtgtacGGTGGGAGGTCTATATAAGCAGTgctagcacactccagagcatcctcgggggtgtcaacaaacactccaccagcattggaaagatctggctcacggtcctcttcatcttccgcatcatgatcctcA(/g)TTgtggctgcaaaggaggtgtggggagatgagcaagccgattttgtctgcaacacgctccagcctggctgcaagaatgtatgctacgaccaccacttcgaattcgccaccA NGG 10 8 17 8 24 0 100 5 10
    241030141633_eaiw acactccagagcatcctcgggggtgtcaacaaacactccaccagcattggaaagatctggctcacggtcctcttcatcttccgcatcatgatcctcA(/G)TTgtggctgcaaaggaggtgtggggagatgagcaagccgattttgtctgcaacacgctccagcctggctgcaagaatgtatgctacgaccaccacttc NGG 10 8 17 8 24 0 100 5 10
    241029152820_5gf8 AGCCCGGCAGTCAAAGCAGAGATCTGGAAATGAACCATCTGCAGAAGGAAATACTGTGGAGGGCTGGCCGGGCAGTCGGGTGCAAGGAAGAAGCCAGAAA(+TTATGTAACAT)CCAGAAGTCTTTTAATATTCCCCCGATGCCACCCGAAAGAGCATTTGAGCTAAGTTTTTTTTTTTACAGAAATGCACTCGGATGCCATCAATAACCATAC NGG 10 8 17 8 24 0 100 5 10
    241029152535_4pj5 GCTACATCAAAAGCCAGAATGAATATTCCAATTAAATTATTGGGCTGATAGTAGCAGGCATGGCTGCTTAATTATCTAGTAAGTCACTAGGGAAGTCCGT(+TTATGTAACAT)CCCCAGGAAGATGGCAGGACACACCATTTTGCTAAAGAGAAAATGCTCAGGAAAACGAAAGCTTAAATACACCCTGGTGTGTCTCGAAAAGGCCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    241029152016_clg2 AGAACTGCATAAACAAGGTATTTATCCATGCAGCTATTGCAGAAGGCAGCTAGGGGAAGCCCCTACCTCTGCTTGTGCTTGCAGGGCCTGCAGTCTGAGA(+TTATGTAACAT)GCTGGAGCCATCTTCCAGCTCAGCCAGTGCCCCCCCCCCCCCCATGTGCCCTGGTGACACTGGGCCCTGCAGCAGAGCGGGGGGTTCACAGGTGAACCCT NGG 10 8 17 8 24 0 100 5 10
    241029125122_8b9z CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241028151711_drl9 GCTACATCAAAAGCCAGAATGAATATTCCAATTAAATTATTGGGCTGATAGTAGCAGGCATGGCTGCTTAATTATCTAGTAAGTCACTAGGGAAGTCCGT(+TTATGTAACAT)CCCCAGGAAGATGGCAGGACACACCATTTTGCTAAAGAGAAAATGCTCAGGAAAACGAAAGCTTAAATACACCCTGGTGTGTCTCGAAAAGGCCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    241025013708_g4hj ctggggtttgacgttcaagccccgccaccaaccctctctctctctgtctttctctcaccctctctcttcagggtgaccggga(/g)ctttccagggcccctgggtgaggctggagagaaggtaagtgcaacctggggggtgccaagggccctggaggatctgggcccaactcagctctgacctcttcttttccatcagggcgaacgtggacccc NGG 10 8 17 8 24 0 100 5 10