Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250107175812_33m7 GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAA(/G)ATATCTGTCAGAATTTGAAGAGATTGAGGGGCACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGA NGG 10 8 17 8 24 0 100 5 10
    250107175530_23qa GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGGCACTGGAAGAAACTTTCCTCCC(/A)AGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGA NGG 10 8 17 8 24 0 100 5 10
    250107170835_j6c9 AAATGTTCTTGGCTGTTTTGTATTGCCTTCTGTGGAGTTTCCAGATCTCTGATGGCCATTTTCCTCGAGCCTGTGCCTCCTCTAAGAACTTGTTGGCAAAAGAATGCTGCCCACCATGGATGGGTGATGGGAGTCCCTGCGGCCAGCTTTCAGGCAGAGGTTCCTGCCAGGATATCCTTCTGTCCAGTGCACCATCTGGACCTCAGTTCCCCTTCAAAG(/A)GGGTGGATGACCGTGAGTCCTGGCCCTCTGTGTTTTATAATAGGACCTGCCAGTGCTCAGGCAACTTCATGGGTTTCAACTGCGGAAACTGTAAGTTTGGATTTGGGGGCCCAAATTGTACAGAGAAGCGAGTCTTGATTAGAAGAAACATTTTTGATTTGAGTGTCTCCGAAAAGAATAAGTTCTTTTCTTACCTCACTTTAGCAAAACATACTATCAGCTCAGTCTATGTCATCCCCACAGGCACCTATGGCCAAATGA NGG 10 8 17 8 24 0 100 5 10
    250107170404_ptlz AAATGTTCTTGGCTGTTTTGTATTGCCTTCTGTGGAGTTTCCAGATCTCTGATGGCCATTTTCCTCGAGCCTGTGCCTCCTCTAAGAACTTGTTGGCAAAAGAATGCTGCCCACCATGGATGGGTGATGGGAGTCCCTGCGGCCAGCTTTCAGGCAGAGGTTCCTGCCAGGATATCCTTCTGTCCAGTGCACCATCTGGACCTCAGTTCCCCTTCAAAGGGGTGGATGACCG(/T)TGAGTCCTGGCCCTCTGTGTTTTATAATAGGACCTGCCAGTGCTCAGGCAACTTCATGGGTTTCAACTGCGGAAACTGTAAGTTTGGATTTGGGGGCCCAAATTGTACAGAGAAGCGAGTCTTGATTAGAAGAAACATTTTTGATTTGAGTGTCTCCGAAAAGAATAAGTTCTTTTCTTACCTCACTTTAGCAAAACATACTATCAGCTCAGTCTATGTCATCCCCACAGGCACCTATGGCCAAATGAACAATGGGTCAACACCCATGTTTAATGATATCAACATCTACGACCTCTTTGTATGGATGCATTACTATGTGTCAAGGGACACACTGCTTGGGGGCTCTGAAATATGGAGGGACATTGATTTTGCCCATGAAGCACCAGGGTTTCTGCCTTGGCACAGACT NGG 10 8 17 8 24 0 50 5 10
    250107165807_kjq8 AAATGTTCTTGGCTGTTTTGTATTGCCTTCTGTGGAGTTTCCAGATCTCTGATGGCCATTTTCCTCGAGCCTGTGCCTCCTCTAAGAACTTGTTGGCAAAAGAATGCTGCCCACCATGGATGGGTGATGGGAGTCCCTGCGGCCAGCTTTCAGGCAGAGGTTCCTGCCAGGATATCCTTCTGTCCAGTGCACCATCTGGACCTCAGTTCCCCTTCAAAGGGGTGGATGACCG(/T)TGAGTCCTGGCCCTCTGTGTTTTATAATAGGACCTGCCAGTGCTCAGGCAACTTCATGGGTTTCAACTGCGGAAACTGTAAGTTTGGATTTGGGGGCCCAAATTGTACAGAGAAGCGAGTCTTGATTAGAAGAAACATTTTTGATTTGAGTGTCTCCGAAAAGAATAAGTTCTTTTCTTACCTCACTTTAGCAAAACATACTATCAGCTCAGTCTATGTCATCCCCACAGGCACCTATGGCCAAATGAACAATGGGTCAACACCCATGTTTAATGATATCAACATCTACGACCTCTTTGTATGGATGCATTACTATGTGTCAAGGGACACACTGCTTGGGGGCTCTGAAATATGGAGGGACATTGATTTTGCCCATGAAGCACCAGGGTTTCTGCCTTGGCACAGACT NGG 10 8 17 8 24 0 100 5 10
    250106033215_3pf4 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250104111020_pzph AGTTGAGAACAAGTTTGGCAGCATCCGGCAGACATACACACTGGATGTGCTGGgtgagggccaggccgggtggctaaggcaaggggcatctctgggtcccttggccctactggccctgacctgactgcctcttgcagAGCGCTC(/G)CCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCAGACAGCCATTCTAGGCAGTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    250104110111_mazm AGTTGAGAACAAGTTTGGCAGCATCCGGCAGACATACACACTGGATGTGCTGGgtgagggccaggccgggtggctaaggcaaggggcatctctgggtcccttggccctactggccctgacctgactgcctcttgcagAGC(/T)GCTCCCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCAGACAGCCATTCTAGGCAGTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    250102071948_maj6 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250102070959_j8wf CAGCCCTTCCTGGACAAGAGCGGCCTGGAGAAGTATCTGTACCCGGCGGCGGCTGCCGCCCC(/G)GTTCCCGCTGCTATACCCCGGCATCCCCGCCCCGGCGGCAGCCGCGGCAGCCGCCGCCGCC NGG 10 8 17 8 24 0 100 5 10