Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241011173558_vii5 CTACTGCTGAAAAGAGAGAGAGTGGACCTAAGCAAGCTGCAGTAAATGCTGCAGTTCAGAGGGTCCAGGTTCTTCCAGATGCTGATACTTTATTACATTT(-t)GCCACGGAAAGTACTCCAGATGGATTTTCTTGTTCATCCAGCCTGAGTGCTCTGAGCCTCGATGAGCCATTTATACAGAAAGATGTGGAATTAAGAATAA NGG 10 8 17 8 24 0 100 5 10
    241011171349_67l9 ATTTAATTGTTTATCATACAGACACTTCATTTGGAGTACCTTAACATGATGTTATCTGTATTTACCTATAGTCTAAATTATACCATCTATAATGTGCTTAa(/g)TTTTTAGGGTTCAACTACACGAATGGACCATGAAACAGCCAGTGTTTTGAGTTCTAGTAGCACACACTCTGCACCTCGAAGGCTGACAAGTCATCTGGGA NG 10 8 17 8 24 0 100 5 10
    241011171033_j6sp ATAGATTTCTATTCTTACTGCTAGCATTAAAAACAAAAAAGCAACTAGTATGATTTTATGTATAAATTAATCTAAAATTGATTAATTTGCAGGTTATTGCG(/A)AGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAATAGTAAAAAGACGTTGCGAGAAGTTGGAAGTGTGAAAGCATTGATGGAATGTGCTTTAGAAG NG 10 8 17 8 24 0 100 5 10
    241011164039_svmq CAGCCTGCGGCCCCCAACACCACCTCCCCCGAGCTCAACCTGTCCCACCCGCTCCTGGGCACCGCCCTGGCCA(/G)ATGGGACAGGTGAGCTCTCGGAGCACCAGCAGTACGTGATCGGCCTGTTC NGG 10 8 17 8 24 0 100 5 10
    241011160232_yj3x CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240914150311_bbdq CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+Agag)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240914150256_m6ry CTTTCGCCAGCCTTACGGGCCCGAACCCTCGTGTGAAGGG(+agaag)TGCAGTACCTAAGCCGGAGCGGGGTAGAGGCGGGCCGGCACCCCCTTCTGACCTCCAGTGCCGCCGGCCTCAAGATCAGACATGGCCCAGAACTTGAAGGACTTGGCGGGACGGCTGCCCGCCGGGCCCCGGGGCATGGGCACGGCCCTGAAGCTGTTGCTGGGGGCCGG NGG 10 8 17 8 24 0 100 5 10
    240914103108_6str CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240913150604_r6x7 GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGG(/A)TGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240913104353_9zuo GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAA(/T)GCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGCTCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10