Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240809085743_wcfz agaactgaagactgtccattcactatggaatgatgagttattggtggtgatgctttggaagtgg(/t)tggaggaggtagtcgaggaggaattgttagtagtgaggtctagccctccatgtttaatgccattgtcttccatactgtg NGG 10 8 17 8 24 0 100 5 10
    240809085713_hfs0 atggaagacaatggcattaaacatggagggctagacctcactactaacaattcctcctcgactacctcctccac(/a)cacttccaaagcatcaccaccaataactcatcattccatagtgaatggacagtctt NGG 10 8 17 8 24 0 100 5 10
    240808200654_0yxy agacaatggcattaaacatggagggctagacctcactactaacaattcctcctcgactacctcctccac(/a)cacttccaaagcatcaccaccaataactcatcattccatagtgaatggacagtcttcagttcta NGG 10 8 17 8 24 0 100 5 10
    240808200633_999p agacaatggcattaaacatggagggctagacctcactactaacaattcctcctcgactacctcctccac(/a)cacttccaaagcatcaccaccaataactcatcattccatagtgaatggacagtcttcagttcta NGG 10 8 17 8 24 0 100 5 10
    240808200305_ig3h agacaatggcattaaacatggagggctagacctcactactaacaattcctcctcgactacctcctccac(/a)cacttccaaagcatcaccaccaataactcatcattccatagtgaatggacagtcttcagttcta NGG 10 8 17 8 24 0 100 5 10
    240808195624_hx4g gtgactggagttcacagtatggaagacaatggcattaaacatggagggctagacctcactactaacaattcctcctcgactacctcctccac(/a)cacttccaaagcatcaccaccaataactcatcattccatagtgaatggacagtcttcagtt NGG 10 8 17 8 24 0 100 5 10
    240807145255_osdj CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240806104538_nivn TACGTTATGTCTAGTTTATTATTATGTAATTTATCTCTTTTTTTTTTCTCCTTTGTTTTGTTTTTAGGTCAATAAGTTTTTATTGTTAGTAATTTGGGAGCTACTGTGTCACAGTGTAATCTCTGTTAT(/a)GGATCCAACGAGGAGTTGGGCAGCTCGATGGCTTCGAATTGGTATGTGAAACCTCTCTTACATATATGTGATTTTTTTAATTGGCCTCTCTTACATTATTATAATTTTGACTAAATTTGGTTACTCGTGTGGTATATATTTTGAGTTTGAAACTTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240806085847_7q67 AGGTCAATAAGTTTTTATTGTTAGTAATTTGGGAGCTACTGTGTCACAGTGTAATCTCTGTTAT(/a)GGATCCAACGAGGAGTTGGGCAGCTCGATGGCTTCGAATTGGTATGTGAAACCTCTCTTACATATATGTGATTTTTTTAATTGGCCTCTCTTACA NGG 10 8 17 8 24 0 100 5 20
    240806085402_uvuy TAATGCAGTTTAGGGAATCAGGATGCGAGAACTGTCCCTTCTTCAAGATGGATGAAGATCATGAGCGTGTAGTGGACTGCACTA(/c)CTCCAAATTTTACTGGGTACGTTATGTCTAGTTTATTATTATGTAATTTATCTCTTTTTTTTTTCTCCTTTGTTTTGTTTTTAGGTCAATAAGTTTTTATTGTTAGTAATTTGGGAGCTACTGTGT NGG 10 8 17 8 24 0 100 5 20