Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240806085141_4dtk TAATGCAGTTTAGGGAATCAGGATGCGAGAACTGTCCCTTCTTCAAGATGGATGAAGATCATGAGCGTGTAGTGGACTGCACTA(/c)CTCCAAATTTTACTGGGTACGTTATGTCTAGTTTATTATTATGTAATTTATCTCTTTTTTTTTTCTCCTTTGTTTTGTTTTTAGGTCAATAAGTTTTTATTGTTAGTAATTTGGGAGCTACTGTGT NGG 10 8 17 8 24 0 100 5 10
    240805164830_ej0u GCGTCTCCTGGAGGATTCGGGCCGCTCTGCCCTGAGGACGTGGTGAGGCCGTGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGAATGGTTCCA(-ATA)GCCTACTTTGCGAAACGGTTTGGATTCACAGAACTTCCGTTGTAAAAGCAATAAGAGAAGTGTCAGAGTTTTCCATTTAGATCAACAACTTCAAATTCTCAGCATGGAGAACTCAGAGAAGACAGAAGTAGTTCTCCTTGCTTGTGGGTCCTTTAATCCTATC NGG 10 8 17 8 24 0 100 5 10
    240805164644_9f3s GCGTCTCCTGGAGGATTCGGGCCGCTCTGCCCTGAGGACGTGGTGAGGCCGTGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGA(-ATG)GTTCCAATAGCCTACTTTGCGAAACGGTTTGGATTCACAGAACTTCCGTTGTAAAAGCAATAAGAGAAGTGTCAGAGTTTTCCATTTAGATCAACAACTTCAAATTCTCAGCATGGAGAACTCAGAGAAGACAGAAGTAGTTCTCCTTGCTTGTGGGTCCTTTAATCCTATC NGG 10 8 17 8 24 0 100 5 10
    240805164404_ds97 GCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCA(-ACG)GAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCAATATCCTCCTGGCCACCGACTCATACAAGGTTACTCACTATAAACAGTACCCACCCAACACA NGG 10 8 17 8 24 0 100 5 10
    240805164139_8now ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTC(-ACG)GCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240805163857_78jv ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCG(-ATG)GGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240805163034_nvve GCGTCTCCTGGAGGATTCGGGCCGCTCTGCCCTGAGGACGTGGTGAGGCCGTGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGAATGGTTCCA(-ATA)GCCTACTTTGCGAAACGGTTTGGATTCACAGAACTTCCGTTGTAAAAGCAATAAGAGAAGTGTCAGAGTTTTCCATTTAGATCAACAACTTCAAATTCTCAGCATGGAGAACTCAGAGAAGACAGAAGTAGTTCTCCTTGCTTGTGGGTCCTTTAATCCTATC NGG 10 8 17 8 24 0 100 5 10
    240805162112_91go GCGTCTCCTGGAGGATTCGGGCCGCTCTGCCCTGAGGACGTGGTGAGGCCGTGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGA(-ATG)GTTCCAATAGCCTACTTTGCGAAACGGTTTGGATTCACAGAACTTCCGTTGTAAAAGCAATAAGAGAAGTGTCAGAGTTTTCCATTTAGATCAACAACTTCAAATTCTCAGCATGGAGAACTCAGAGAAGACAGAAGTAGTTCTCCTTGCTTGTGGGTCCTTTAATCCTATC NGG 10 8 17 8 24 0 100 5 10
    240805121815_fxds GCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCA(-ACG)GAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGT NGG 10 8 17 8 24 0 100 5 10
    240805115148_arcu ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGATGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTC(-ACG)GCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTCA NGG 10 8 17 8 24 0 100 5 10