Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144319_kj9g CAAGCCCTGACCTGGAGTCCTGGTTCATCCTG(+AGGACA)GTACCACCCACAATGAAGACAGATCTGCTCG NGG 10 8 17 8 24 0 100 5 10
    240613144317_o3fk CCCATCGAGCAGATCTGTCTTCATTGTGGGTG(+AGGACA)GTACCAGGATGAACCAGGACTCCAGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613144316_aw1o CCCCTCCTCTCCTTACAGCTGGACGTCATCCG(+AGGACA)GATCCAGCTCTCTTAGACAGTTAATCCTAAG NGG 10 8 17 8 24 0 100 5 10
    240613144315_bap5 TCAAACTTAGGATTAACTGTCTAAGAGAGCTG(+AGGACA)GATCCGGATGACGTCCAGCTGTAAGGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613144313_j5fn ATTGCATCCCCAAAAGTTGTTATTTTGTTTCG(+AGGACA)GATCCCACATTTCCCTTGCCTCCATATGGTT NGG 10 8 17 8 24 0 100 5 10
    240613144312_baw3 CCAGGAACCATATGGAGGCAAGGGAAATGTGG(+AGGACA)GATCCGAAACAAAATAACAACTTTTGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240613144310_mvqv AATGGAGACATCCGAAGGAGCCCGTTAACTGG(+AGGACA)GTACCCTTTCTGAAGCTCACTTTTGGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240613144308_b2v9 AATCATGGACCCAAAAGTGAGCTTCAGAAAGG(+AGGACA)GTACCCAGTTAACGGGCTCCTTCGGATGTCT NGG 10 8 17 8 24 0 100 5 10
    240613144307_njg8 GGCCACTGGGCAGAAAGGCTTTATGCCACTTG(+AGGACA)GTACCTTGGTAACACACTTCAAAAGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240613144305_odzz TGGGGTTGCTTCTTTTGAAGTGTGTTACCAAG(+AGGACA)GTACCAAGTGGCATAAAGCCTTTCTGCCCAG NGG 10 8 17 8 24 0 100 5 10