Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144246_hkav GGTGTCAGCGTTTGCATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613144243_4kx0 GGATGGACCATGTAGAGACTGCCATATCCAGG(+AGGACA)GATCCACCCCATAATCAGCATGCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613144242_d24i ATGTTTATAATTTTATATGAGCTACATTCTAG(+AGGACA)GTACCCTTAAACAAGTTTGATACATGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240613144240_5nt4 ACCCATGACCCATGTATCAAACTTGTTTAAGG(+AGGACA)GTACCTAGAATGTAGCTCATATAAAATTATA NGG 10 8 17 8 24 0 100 5 10
    240613144239_a3yk ATTTTGCCATGTGGCTGGTTACCTGTGTTTGG(+AGGACA)GTACCACACATCAGTCTCCTCATGTCTTACC NGG 10 8 17 8 24 0 100 5 10
    240613144237_dyrx CGCCTGGTAAGACATGAGGAGACTGATGTGTG(+AGGACA)GTACCCAAACACAGGTAACCAGCCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240613144236_o3tv GTAACGCCCCAGCCGCAAGGCTGCCTCGCTGG(+AGGACA)GTACCCATGTGGCAACCAGCGATGTGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240613144234_qbsl GCAACCTGACCACATCGCTGGTTGCCACATGG(+AGGACA)GTACCCAGCGAGGCAGCCTTGCGGCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613144233_8ig1 CTGGCTTCTGAAACAAGCAAACCCCTTTAAAG(+AGGACA)GTACCCTGGGAAGTCAAGTTTGCTAGCAGTC NGG 10 8 17 8 24 0 100 5 10
    240613144230_ot0x TCTCAGACTGCTAGCAAACTTGACTTCCCAGG(+AGGACA)GTACCTTTAAAGGGGTTTGCTTGTTTCAGAA NGG 10 8 17 8 24 0 100 5 10