Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144147_4eyk TGTTTTTCTGTTTGTGTTCACTCCTTTTCTAG(+AGGACA)GATCCAGGCAAAGGTGTAAGCCGAGCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613144146_q9hy TTGAGTTAAAGCTCGGCTTACACCTTTGCCTG(+AGGACA)GATCCTAGAAAAGGAGTGAACACAAACAGAA NGG 10 8 17 8 24 0 100 5 10
    240613144145_ogw7 TTCTCGCTCCCTGGAGAGCCTGGAAGACACTG(+AGGACA)GATCCTCAGAGAGACAAGGCGGCGTGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240613144144_80cw AGGCTGTCAGCACGCCGCCTTGTCTCTCTGAG(+AGGACA)GATCCAGTGTCTTCCAGGCTCTCCAGGGAGC NGG 10 8 17 8 24 0 100 5 10
    240613144142_4lx2 GTAAATGCTAACAGGGGTGGGAGGCTCTGGTG(+AGGACA)GATCCTGACAGACACAACCCATAGACACTGC NGG 10 8 17 8 24 0 100 5 10
    240613144141_x3vw TGGTTGCAGTGTCTATGGGTTGTGTCTGTCAG(+AGGACA)GATCCACCAGAGCCTCCCACCCCTGTTAGCA NGG 10 8 17 8 24 0 100 5 10
    240613144139_gtv3 TCTCTGTTAAGTCGATGTGGACTCCAGTCCAG(+AGGACA)GTACCTGAATGGTTTACCTACGCACAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613144138_x93y CACCGGGCTCTGTGCGTAGGTAAACCATTCAG(+AGGACA)GTACCTGGACTGGAGTCCACATCGACTTAAC NGG 10 8 17 8 24 0 100 5 10
    240613144137_jt2n TTAGGATCTAGACCTCTCCTCTGAGCGCTCTG(+AGGACA)GATCCAAATCTTAGCACCAGCTTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613144136_h7h8 GCGAAGAAGGAAGAAGCTGGTGCTAAGATTTG(+AGGACA)GATCCAGAGCGCTCAGAGGAGAGGTCTAGAT NGG 10 8 17 8 24 0 100 5 10