Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144117_mgv7 GTGCACACAGGACAACGGTGCTGTAGCAAGGG(+AGGACA)GTACCCAGGAAAGCAAACTACTCCATGTCCG NGG 10 8 17 8 24 0 100 5 10
    240613144115_8zlz CATGAGGAGCACACAAGAGGGCCTCCTCAGGG(+AGGACA)GTACCCTGCTCCAGCCCCCAGGCTTTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613144113_9orh AAGTGTTAAAAAAGCCTGGGGGCTGGAGCAGG(+AGGACA)GTACCCCTGAGGAGGCCCTCTTGTGTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613144112_ldnk GGCAGGGGTGGGAGTGAGGCACAGAAGCGTGG(+AGGACA)GATCCCCAGCTAGGAATGTGGGTGCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613144110_5j7n CTTATGAGCAAGCACCCACATTCCTAGCTGGG(+AGGACA)GATCCCACGCTTCTGTGCCTCACTCCCACCC NGG 10 8 17 8 24 0 100 5 10
    240613144107_7d07 TCACACAACCATTTATAACTCTGGTTCCAGGG(+AGGACA)GATCCCACACCCTATTCTGACCTCTACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613144106_rorg TCAGTGCCTGTAGAGGTCAGAATAGGGTGTGG(+AGGACA)GATCCCCTGGAACCAGAGTTATAAATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613144103_uv8w TAGATCAGGCTCTCTAGCTAGGAAGCCCTTGG(+AGGACA)GATCCTCCTGACTCTTAAGCACTGAGATTAC NGG 10 8 17 8 24 0 100 5 10
    240613144102_qwr6 CACTTGTAATCTCAGTGCTTAAGAGTCAGGAG(+AGGACA)GATCCCAAGGGCTTCCTAGCTAGAGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613144100_wnjh CCCTCAGCCCTCAACCCCTGCTCACCCCTCAG(+AGGACA)GATCCTGCTGCACTCAGCTTCCGCAGGAACA NGG 10 8 17 8 24 0 100 5 10