Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144059_23wi GCTGCTGTTCCTGCGGAAGCTGAGTGCAGCAG(+AGGACA)GATCCTGAGGGGTGAGCAGGGGTTGAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613144057_r9e9 CTGCTGTCCCGACTCCTGCACAGGCTGAGGTG(+AGGACA)GTACCAAGATGGCCATCTGTGGCTACTCACC NGG 10 8 17 8 24 0 100 5 10
    240613144056_uaam TTGCCGGTGAGTAGCCACAGATGGCCATCTTG(+AGGACA)GTACCACCTCAGCCTGTGCAGGAGTCGGGAC NGG 10 8 17 8 24 0 100 5 10
    240613144054_91xz AGGGGACGGCCAGACAGTACAGTCACCCATAG(+AGGACA)GATCCCCAGAGACATAAAGGGTATCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240613144049_18cx CTTTCTGTCCTGATACCCTTTATGTCTCTGGG(+AGGACA)GATCCTATGGGTGACTGTACTGTCTGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240613144047_h36m CACCACCGGGATCTGTCCTGCAAAGTTGAAGG(+AGGACA)GATCCTTTGGTGCCAGCTGGTGTTTGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240613144045_o7bz TTCCTACTTCCAAACACCAGCTGGCACCAAAG(+AGGACA)GATCCCTTCAACTTTGCAGGACAGATCCCGG NGG 10 8 17 8 24 0 100 5 10
    240613144043_6p6u CCTCTCATGGCGCTGCTACCGATGACTCCCAG(+AGGACA)GATCCCAGACGTTCAGAACCAGATTCTCATT NGG 10 8 17 8 24 0 100 5 10
    240613144041_oxvb AAAGCAATGAGAATCTGGTTCTGAACGTCTGG(+AGGACA)GATCCTGGGAGTCATCGGTAGCAGCGCCATG NGG 10 8 17 8 24 0 100 5 10
    240613144039_rm0v CCTGGCAGGCAGGTGGGCGGTCTGCCTCCAGG(+AGGACA)GATCCTGGGACCCTGATGGCGATCGTCCTGT NGG 10 8 17 8 24 0 100 5 10