Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143943_wfgh TACAGGCTTTATAGAAAGTAAAGTAGCATTGG(+AGGACA)GTACCCTTGAGCAATATCTTGGGGTTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613143942_k1f5 CTGAAAATATGAAGAATTATTTTTATACAAAG(+AGGACA)GATCCTTAAGCTTGGAGCACAATAAAGATGA NGG 10 8 17 8 24 0 100 5 10
    240613143941_iow7 AGAGGTCATCTTTATTGTGCTCCAAGCTTAAG(+AGGACA)GATCCTTTGTATAAAAATAATTCTTCATATT NGG 10 8 17 8 24 0 100 5 10
    240613143939_qbqj AAGCACTGAGTGCCATTAGCTAAATGCATAGG(+AGGACA)GTACCACCCACAGGTGCCAGGGGCCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613143938_0s47 ACTTTGGGAAAGGCCCCTGGCACCTGTGGGTG(+AGGACA)GTACCCTATGCATTTAGCTAATGGCACTCAG NGG 10 8 17 8 24 0 100 5 10
    240613143936_hscg TTTGGATCTTTAGTCAAATTGGCTAAGACTTG(+AGGACA)GATCCCAGAACCCAAGAAGAAAGGAAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613143935_ewnp TTGGGTCCCCTTCCTTTCTTCTTGGGTTCTGG(+AGGACA)GATCCAAGTCTTAGCCAATTTGACTAAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613143933_ulie CCATCTTGTCTCTTCCCTACTGTCTTCAGTGG(+AGGACA)GATCCCCTCTTGGGTCCCTCCCTTTGTCATC NGG 10 8 17 8 24 0 100 5 10
    240613143931_n2xr CACATGATGACAAAGGGAGGGACCCAAGAGGG(+AGGACA)GATCCCACTGAAGACAGTAGGGAAGAGACAA NGG 10 8 17 8 24 0 100 5 10
    240613143930_at4x GTAGGTCGAGCTAGAGGGCTACAGAGAGGCAG(+AGGACA)GATCCAGGATTAGAGAAATTCCTAAGATCTG NGG 10 8 17 8 24 0 100 5 10