Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613145348_opt3 TCAGAGTCTGCAACTTTATGAGGAACTAAAAG(+AGGACA)GTACCCGGAAACCTCACACACTAGAAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613145347_vz7r AAAAGGTTTGTTTTCTTTCATAATTGCATCTG(+AGGACA)GTACCCAGCAGGAAGTACCTGGCCCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240613145345_a8t7 CGAACAAGGAGGGGCCAGGTACTTCCTGCTGG(+AGGACA)GTACCAGATGCAATTATGAAAGAAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240613145342_h21w TGTTATGAAATATAATCTGGTGGCCGGCCGGG(+AGGACA)GTACCTGGAGGACCCATGCCAAGCTGTGCAA NGG 10 8 17 8 24 0 100 5 10
    240613145340_oeni CTCAGTTGCACAGCTTGGCATGGGTCCTCCAG(+AGGACA)GTACCCCGGCCGGCCACCAGATTATATTTCA NGG 10 8 17 8 24 0 100 5 10
    240613145339_1m37 CCTTTGACCATCTCTGTTTTGGGTTCAGTTTG(+AGGACA)GATCCTGAAACTTTCCTTCCCAGAAGGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613145338_fp74 GGGCACAAACCTTCTGGGAAGGAAAGTTTCAG(+AGGACA)GATCCAAACTGAACCCAAAACAGAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613145336_vmr4 AAATCACTGACTGTATCCCTGGGGTTGTTCTG(+AGGACA)GATCCTCTGCATCAGGTCACCTCATGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613145335_r9r8 CTCCCTCACCCATGAGGTGACCTGATGCAGAG(+AGGACA)GATCCAGAACAACCCCAGGGATACAGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240613145334_ni8l GTCTCACTCCCTGCCAGCGGCAGTGCTGACAG(+AGGACA)GATCCTCCTCAAAGGTGCTTAGCTTTGGCAA NGG 10 8 17 8 24 0 100 5 10