Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143928_6pti TCTGGCAGATCTTAGGAATTTCTCTAATCCTG(+AGGACA)GATCCTGCCTCTCTGTAGCCCTCTAGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240613143926_8qwr CTTTGCCCATGTATCTGCTCCTACAGCGGGTG(+AGGACA)GTACCCACATCACTCAAGTACATATCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240613143924_0itp GTGACAGCCGGATATGTACTTGAGTGATGTGG(+AGGACA)GTACCACCCGCTGTAGGAGCAGATACATGGG NGG 10 8 17 8 24 0 100 5 10
    240613143923_6t3h ACACTCAATGCACTTTATTACACAAAATGAGG(+AGGACA)GATCCGTAAACAGGGCAGGACCCAAGAGTCC NGG 10 8 17 8 24 0 100 5 10
    240613143921_19vz GCTCAGGACTCTTGGGTCCTGCCCTGTTTACG(+AGGACA)GATCCCTCATTTTGTGTAATAAAGTGCATTG NGG 10 8 17 8 24 0 100 5 10
    240613143920_rdfx GTAGGTCGAGCTAGAGGGCTACAGAGAGGCAG(+AGGACA)GATCCAGGATTAGAGAAATTCCTAAGATCTG NGG 10 8 17 8 24 0 100 5 10
    240613143918_bsr3 TCTGGCAGATCTTAGGAATTTCTCTAATCCTG(+AGGACA)GATCCTGCCTCTCTGTAGCCCTCTAGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240613143917_k3ml GCAGTACTCTGAAGCAGGCAAATGGGTCTTAG(+AGGACA)GATCCCTCCCAGAAACCTGCTCTGACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613143915_lqr2 ATGGGTCTGGGTCAGAGCAGGTTTCTGGGAGG(+AGGACA)GATCCTAAGACCCATTTGCCTGCTTCAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613143914_7syq TCCCATGGGAATTTTCGTCTGTTAATACTGTG(+AGGACA)GATCCGGTCTGTTTATATGGGGAATGTGCTG NGG 10 8 17 8 24 0 100 5 10