Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143824_wtmq GCTGAAGGGGACGGAGGAGCTCGTATATACAG(+AGGACA)GATCCATCATGATTAACTGCATACTGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613143822_hgeq GGGAGGCTGTAAAATGGCCCATGTATATGTAG(+AGGACA)GATCCTGGATGGTCTACATATATGTTAATAC NGG 10 8 17 8 24 0 100 5 10
    240613143820_fbrs TTCATGTATTAACATATATGTAGACCATCCAG(+AGGACA)GATCCTACATATACATGGGCCATTTTACAGC NGG 10 8 17 8 24 0 100 5 10
    240613143819_t66s GGACACCCTGTAACCTTACTGCTTCAGGGCTG(+AGGACA)GATCCAGACTAAGAGGTTGTGAGGTTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613143818_fi9o CTGCATCCCCAACCTCACAACCTCTTAGTCTG(+AGGACA)GATCCAGCCCTGAAGCAGTAAGGTTACAGGG NGG 10 8 17 8 24 0 100 5 10
    240613143817_ee0u TTATAGGGAAGATGATGTTAACACCTACTTTG(+AGGACA)GTACCTTGACAAGTGTTACAGAGATCAACAA NGG 10 8 17 8 24 0 100 5 10
    240613143816_ywg5 CCCTTTTGTTGATCTCTGTAACACTTGTCAAG(+AGGACA)GTACCAAAGTAGGTGTTAACATCATCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613143814_x7xa GCTCCTTCCCTGTATCTCTCAGGCAGCAAGTG(+AGGACA)GATCCCTCCGGACTAAAGGCCTAAGGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613143812_k6p8 GTCCCTGAAACCTTAGGCCTTTAGTCCGGAGG(+AGGACA)GATCCACTTGCTGCCTGAGAGATACAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613143811_8b96 TTTATAGCAAGAACAGTTCCCACTCCGAGCAG(+AGGACA)GATCCTAAACTCCATCCCAAGTGAATACAGA NGG 10 8 17 8 24 0 100 5 10