Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143810_qj4t AGAGATCTGTATTCACTTGGGATGGAGTTTAG(+AGGACA)GATCCTGCTCGGAGTGGGAACTGTTCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240613143808_rnl6 CCAGGCCGTGCTGGGGTGCTCTCTCTGCTGGG(+AGGACA)GATCCTTATGGGGCCGGAGGCCACCTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613143807_fekk ATGCTTTCCTAGGTGGCCTCCGGCCCCATAAG(+AGGACA)GATCCCCAGCAGAGAGAGCACCCCAGCACGG NGG 10 8 17 8 24 0 100 5 10
    240613143805_vjma TCTATGAGTATCTGTAAATGTTTTCTGAAGAG(+AGGACA)GATCCACTGAGCAGCTGTAACCCATCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613143804_tba8 CCACACTGGAGATGGGTTACAGCTGCTCAGTG(+AGGACA)GATCCTCTTCAGAAAACATTTACAGATACTC NGG 10 8 17 8 24 0 100 5 10
    240613143803_88w2 GTTCAAGGTCAGAGAGGTCTGGGTGTGGGCTG(+AGGACA)GATCCCATGCAGATGTTTGGTAGGTTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613143801_jgs3 TGGGGTGCTCAACCTACCAAACATCTGCATGG(+AGGACA)GATCCAGCCCACACCCAGACCTCTCTGACCT NGG 10 8 17 8 24 0 100 5 10
    240613143800_pejg GGGGAGGTGAGACAGCTCAAATTTTGATCATG(+AGGACA)GATCCAACCTCACGGGCACTAGACAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613143759_izg8 GTGAAACACTCTGTCTAGTGCCCGTGAGGTTG(+AGGACA)GATCCATGATCAAAATTTGAGCTGTCTCACC NGG 10 8 17 8 24 0 100 5 10
    240613143757_qgjt CAATCCTATCATGCAAGATTGAGGCCCTTAGG(+AGGACA)GTACCTGACAACGGGCACTAATACACCCCAT NGG 10 8 17 8 24 0 100 5 10