Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143709_ebea TGGTGCCCCTGGATCTATGGCTCAGCCAATTG(+AGGACA)GTACCTGCAGCAAGGGCTCGCCCCCTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613143708_g0aa AAGCTTTCTTAGAACTTGTTTTTATTCTTTGG(+AGGACA)GTACCCAAAGGCCAAGTGATGAATCTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240613143706_0rld AATTCTTGCCAGATTCATCACTTGGCCTTTGG(+AGGACA)GTACCCAAAGAATAAAAACAAGTTCTAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613143704_8ocj TGTGGGAGAAGAGCCCTGACCAAGCTGATCCG(+AGGACA)GTACCTTGAAGGAAGTGCAATTGTTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240613143703_ezoz AGTATGCATGAAACAATTGCACTTCCTTCAAG(+AGGACA)GTACCGGATCAGCTTGGTCAGGGCTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613143702_tib3 AGCTAGAGTCTGGGTTGGAGAGGCCGAGTGAG(+AGGACA)GATCCTTACAAGGCTACTGCATAGCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613143701_g3jl TCCCGTGAGAAGCTATGCAGTAGCCTTGTAAG(+AGGACA)GATCCTCACTCGGCCTCTCCAACCCAGACTC NGG 10 8 17 8 24 0 100 5 10
    240613143700_fir4 CACAGAAGTTATCTTGACCAAATGGCCTATTG(+AGGACA)GTACCTACTCTTAATTTATTCTTTCAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613143658_uar2 GGGGAGTGCTTGAAAGAATAAATTAAGAGTAG(+AGGACA)GTACCAATAGGCCATTTGGTCAAGATAACTT NGG 10 8 17 8 24 0 100 5 10
    240613143657_28uq AAATAGCTCCCCACCTTCCCAAGGCTGTTATG(+AGGACA)GTACCAGACACACAGCTCAGGACACCCCAGG NGG 10 8 17 8 24 0 100 5 10