Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143639_l94w ATTTTAACCACATCAGTGGAAATGGGTTGCAG(+AGGACA)GATCCAGGGGTGAGTTCTAACTGCTGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240613143637_61ih GTCACCCCAAACCAAAGCGAAACACAGACCTG(+AGGACA)GTACCGAAGACACTTCGTATTCCCAAAGCCG NGG 10 8 17 8 24 0 100 5 10
    240613143636_kl8w GCACACGGCTTTGGGAATACGAAGTGTCTTCG(+AGGACA)GTACCAGGTCTGTGTTTCGCTTTGGTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240613143635_kp1j GTTTGTCTGGGTGTAGGGTTGGACAGCTCTGG(+AGGACA)GATCCGATTAAGGCCATGTTCTGGGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613143633_1at7 TGAGGGGCAGCCCCAGAACATGGCCTTAATCG(+AGGACA)GATCCCAGAGCTGTCCAACCCTACACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613143632_n6nq ATGCTGCCTGTGAAGCTGTGTTCTCAGGTCAG(+AGGACA)GATCCTGTTCACCAGCGTCAGAAAGTGTACA NGG 10 8 17 8 24 0 100 5 10
    240613143631_a56v TGGTGTGTACACTTTCTGACGCTGGTGAACAG(+AGGACA)GATCCTGACCTGAGAACACAGCTTCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613143630_9tcl CACGCGCCGCTCTGCTCCGGAGCTCTGCGAAG(+AGGACA)GATCCCGACCGCGCCGGGCTATGCAGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613143628_hctr CTGCAGCTGCCTGCATAGCCCGGCGCGGTCGG(+AGGACA)GATCCTTCGCAGAGCTCCGGAGCAGAGCGGC NGG 10 8 17 8 24 0 100 5 10
    240613143627_5lk5 AGTCTCCAGATTCAGGTAACAGCTTGATATGG(+AGGACA)GATCCATAAGTGTCAAATTGGGCTTTGTGTG NGG 10 8 17 8 24 0 100 5 10