Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143537_f97r GCTCACAATTGCTTGCGATTCCAGTTCCAGGG(+AGGACA)GATCCAGCTCCCTCTGTGAACACTACACTGA NGG 10 8 17 8 24 0 100 5 10
    240613143536_rdp2 CTGGAGTGGGAGGAGAATTCACATCCTCGGAG(+AGGACA)GATCCAGAGAGGCCCAGCGACCATGCTGCAC NGG 10 8 17 8 24 0 100 5 10
    240613143535_a3fl AAGATGTGCAGCATGGTCGCTGGGCCTCTCTG(+AGGACA)GATCCTCCGAGGATGTGAATTCTCCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613143533_sgrx TTTGGCTTCTTTGGAGATGTCAGAGGCCTCAG(+AGGACA)GATCCAGTTCCCGGCGTCTTTGAGCCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613143532_53tm TGGCTGGCTGGGCTCAAAGACGCCGGGAACTG(+AGGACA)GATCCTGAGGCCTCTGACATCTCCAAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240613143530_dhvt GCAGCCCCCAAGAATAGCATCTATACAGGCTG(+AGGACA)GTACCCCAGGTTTCCACAGGAAACAGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613143528_txw0 CTGCCAGCAGCTGTTTCCTGTGGAAACCTGGG(+AGGACA)GTACCAGCCTGTATAGATGCTATTCTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613143525_545l CAGGCGGGCCTCTTTCCTCTCATTTAACCCAG(+AGGACA)GATCCTTAGGCTGAAACAGTTCCCCAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613143523_6xui CTGCTCTGCTTGGGGAACTGTTTCAGCCTAAG(+AGGACA)GATCCTGGGTTAAATGAGAGGAAAGAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240613143522_693a AAGAGAGCTAGGGGTGGGACAGTCCAAACTGG(+AGGACA)GTACCCATAGCCCAGTCTCGGTCAAGCTGGT NGG 10 8 17 8 24 0 100 5 10