Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143446_pajw GAGTCTCAGTCCCCTGGAGGCTACAGAAGAAG(+AGGACA)GATCCTCTGAGGTCAGGGCAACACATGCAAA NGG 10 8 17 8 24 0 100 5 10
    240613143443_pbqv CTATATCCCTGGACCCTAGACAAGAGGACCCG(+AGGACA)GATCCACCTGTGGGCTTCTAGCATTACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613143441_g9le TAACTCTGGGTAATGCTAGAAGCCCACAGGTG(+AGGACA)GATCCGGGTCCTCTTGTCTAGGGTCCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613143438_mu7p CTCTGCCTTCACCTGCAGACACCCTCCCTCCG(+AGGACA)GTACCCTGGGCTTCTACCCTCCCTAGAATTC NGG 10 8 17 8 24 0 100 5 10
    240613143435_kxew GATGGGAATTCTAGGGAGGGTAGAAGCCCAGG(+AGGACA)GTACCGGAGGGAGGGTGTCTGCAGGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613143433_80v4 CCCGCGCTGGACAGCCCAGCGCCCAATCCGGG(+AGGACA)GATCCAGAGGGTAGGCCTGCCCTTGCACTTA NGG 10 8 17 8 24 0 100 5 10
    240613143431_s94t TGGTTTAAGTGCAAGGGCAGGCCTACCCTCTG(+AGGACA)GATCCCCGGATTGGGCGCTGGGCTGTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613143430_o1ap GTTAACTGTTACAGAGGAACTGGCTTTCTGAG(+AGGACA)GTACCTGGCATCTTGGGACGAGTGAAATCTA NGG 10 8 17 8 24 0 100 5 10
    240613143428_l8x7 CTGACTAGATTTCACTCGTCCCAAGATGCCAG(+AGGACA)GTACCTCAGAAAGCCAGTTCCTCTGTAACAG NGG 10 8 17 8 24 0 100 5 10
    240613143426_db6q GGATCTAGCTGGCCCCTTCCCTCCCCAGCCTG(+AGGACA)GTACCCCAGGTCCAATGACATCAGTCTGACT NGG 10 8 17 8 24 0 100 5 10