Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143334_aqbd GATCTGCATGAACCACGCTGTGGGGTCTCTGG(+AGGACA)GTACCTCCCTGGCATCTGGCCATAGGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613143333_571q GGTGACAGCTCAGTGTATTAGTGCTGGGTGTG(+AGGACA)GTACCTGCTCTGGAGTCAGACTGCCTAGCAT NGG 10 8 17 8 24 0 100 5 10
    240613143331_vqtn ATCTGATGCTAGGCAGTCTGACTCCAGAGCAG(+AGGACA)GTACCACACCCAGCACTAATACACTGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613143329_pwqp TAGGAAGGGCTTTGTGAAGACACGTCACAGGG(+AGGACA)GTACCTGACAGAATCTGCTCCTCTTACTCTA NGG 10 8 17 8 24 0 100 5 10
    240613143328_nfl7 TGCCCTAGAGTAAGAGGAGCAGATTCTGTCAG(+AGGACA)GTACCCCTGTGACGTGTCTTCACAAAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240613143326_3n75 CTGCCTCATGTGAGTGCTGAGAACCAAACCTG(+AGGACA)GATCCTTTGTAAGAGCTGCCACTGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613143325_wf3a AATGGTTAAGAGCAGTGGCAGCTCTTACAAAG(+AGGACA)GATCCAGGTTTGGTTCTCAGCACTCACATGA NGG 10 8 17 8 24 0 100 5 10
    240613143324_7zpm AGTGTCAGGCCATGGAGCCCACCCCTGAGCTG(+AGGACA)GATCCCACCTTGGGCTGTCGCTGAACCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613143322_oigh GGAAAAGGAGGTTCAGCGACAGCCCAAGGTGG(+AGGACA)GATCCAGCTCAGGGGTGGGCTCCATGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613143321_dfry ATGACTGTTGGAAATCAAAGACCAGAGAGACG(+AGGACA)GTACCTCGTAACCATGGAGCTGGAAAGCTCT NGG 10 8 17 8 24 0 100 5 10