Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143252_7eru AGCTCACACCTGTCTGTAATTCCAGTTTTAAG(+AGGACA)GATCCGATGCTCTCCCACAACCATGCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240613143251_0bix TTGGCTGCGTCTTTCGCCAAGCTAGGCTACTG(+AGGACA)GTACCTGCCTTGGGCCGTGGATGGGGTGATA NGG 10 8 17 8 24 0 100 5 10
    240613143249_lzbs CTTCTTATCACCCCATCCACGGCCCAAGGCAG(+AGGACA)GTACCAGTAGCCTAGCTTGGCGAAAGACGCA NGG 10 8 17 8 24 0 100 5 10
    240613143247_kff8 GGGTGTGGTCTCCAGGCCAGATCAGGTTATGG(+AGGACA)GATCCCATAGATTATGTACGTAGACTGCACA NGG 10 8 17 8 24 0 100 5 10
    240613143246_45kc ACCAATGTGCAGTCTACGTACATAATCTATGG(+AGGACA)GATCCCATAACCTGATCTGGCCTGGAGACCA NGG 10 8 17 8 24 0 100 5 10
    240613143245_85g3 AGACACTGCTCATGTGACCTTCTACCTTTGTG(+AGGACA)GATCCCTCTTGGTTTGTGACCTTCGTACCTT NGG 10 8 17 8 24 0 100 5 10
    240613143243_q3lg CAGGGAAGGTACGAAGGTCACAAACCAAGAGG(+AGGACA)GATCCACAAAGGTAGAAGGTCACATGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613143241_97mo TAGCAGCTCACAACTGTAACTTCAATTCTGGG(+AGGACA)GATCCCATGTCTTTTTCTGACCTCCACAGGG NGG 10 8 17 8 24 0 100 5 10
    240613143239_ro9l TAAGTCCCTGTGGAGGTCAGAAAAAGACATGG(+AGGACA)GATCCCCAGAATTGAAGTTACAGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613143238_ak6f CAACATATGTAAAGCCGTGGGTTGAACCTATG(+AGGACA)GATCCCTCAGCCTCCAAAAGAGGCAGATGCT NGG 10 8 17 8 24 0 100 5 10