Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143205_3eht CACAGGGGCAGGCTTCACAGCATCAGTGCATG(+AGGACA)GATCCACAAACTATGTTACAGCTAGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240613143204_d5g6 CAAGCTGTCGAGAAATGGTTCTCTTTACAGTG(+AGGACA)GATCCAGTCAGGATTGGCAGCAAGCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240613143202_jxze CAGGCAAAGGTGCTTGCTGCCAATCCTGACTG(+AGGACA)GATCCACTGTAAAGAGAACCATTTCTCGACA NGG 10 8 17 8 24 0 100 5 10
    240613143201_dc2n GCAAACAGCAAGAGGCTTTATTGAGAACACGG(+AGGACA)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240613143158_hvbp GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+AGGACA)GTACCCGTGTTCTCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613143157_dz98 TAAGAGAAAACTGACAAACTACTTAATGAAGG(+AGGACA)GTACCAAGAAAGGACAATCGGATTCAGTTCG NGG 10 8 17 8 24 0 100 5 10
    240613143155_qohi TCTAGCGAACTGAATCCGATTGTCCTTTCTTG(+AGGACA)GTACCCTTCATTAAGTAGTTTGTCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613143154_6l6r GAGCTGCCAGCTCCTAGGGACAGATGCTAGAG(+AGGACA)GTACCCACAGCTCCCCTGAAGACCTCGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613143152_sixj ACTGAGAAACGAGGTCTTCAGGGGAGCTGTGG(+AGGACA)GTACCTCTAGCATCTGTCCCTAGGAGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613143150_sdwq TTTTCTTTGTTGAAACTCTATGAATACCACAG(+AGGACA)GTACCTACGTGGCCCATGGCTGGTAACTATT NGG 10 8 17 8 24 0 100 5 10