Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143107_hpfq CAATGCGACCATATGGAAATGAGTCACTGCAG(+AGGACA)GATCCAATGAAAATGAGCACAAGTTACTTAG NGG 10 8 17 8 24 0 100 5 10
    240613143105_td25 GCTACATCTCCCAGACTTTCAGTCTTATCTAG(+AGGACA)GATCCTCTCCTCTTCCCAGCCTGAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240613143104_br3z TGGGGCCTGGGTTCAGGCTGGGAAGAGGAGAG(+AGGACA)GATCCTAGATAAGACTGAAAGTCTGGGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613143102_wnln TAATTGTATTTGGTTGTTTGTGCTTGCAGGTG(+AGGACA)GTACCACCTAGGCAAATGTCTGACCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613143101_5i34 CTATGAGGAGGGGTCAGACATTTGCCTAGGTG(+AGGACA)GTACCACCTGCAAGCACAAACAACCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240613143059_9y17 CAAAAAACCAGGCCATTCCTTCACTTCAAAGG(+AGGACA)GTACCACTTTCCTTATGGATGGACCAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240613143058_456k ACGTGATTCCTGGTCCATCCATAAGGAAAGTG(+AGGACA)GTACCCTTTGAAGTGAAGGAATGGCCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613143056_t39g TCAATCTTGTTTTACAGCTTATTTCAGAGAGG(+AGGACA)GATCCTGGAAATATTTACAGAGTTTTTTTAG NGG 10 8 17 8 24 0 100 5 10
    240613143054_fzwm TAAGGCTAAAAAAACTCTGTAAATATTTCCAG(+AGGACA)GATCCCTCTCTGAAATAAGCTGTAAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240613143052_gk54 GAGCCTCTGTACAGGAATCTTCAAATAACATG(+AGGACA)GTACCCCTAAAACTGAGAATTGTTTCTAGGC NGG 10 8 17 8 24 0 100 5 10