Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143024_once TGTGTATATATGTGTATATATATACACACATG(+AGGACA)GTACCTTATTCATTTGGGGTCTTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613143023_fnms TTTAGTCACAATAAGGACCCCAAATGAATAAG(+AGGACA)GTACCATGTGTGTGTGTGTGTGTGTGTATAC NGG 10 8 17 8 24 0 100 5 10
    240613143021_m60i TATGTGTATACACACACACACACACACACATG(+AGGACA)GTACCTTATTCATTTGGGGTCCTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613143020_w0s5 TAGTGTTTAAAGCATGTGATGCAGTGGCAGAG(+AGGACA)GATCCAGGTTTAGTTCCTAGCACCTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240613143018_srxj AGGTGCCACACAGGTGCTAGGAACTAAACCTG(+AGGACA)GATCCTCTGCCACTGCATCACATGCTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613143016_vdqn GTGGTAAGTGATTAACCATGTTCTGGTGGCGG(+AGGACA)GTACCAAACTGTTCTTCATGATAGAGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240613143015_8y0o GAAGCAGCTACTCTATCATGAAGAACAGTTTG(+AGGACA)GTACCCGCCACCAGAACATGGTTAATCACTT NGG 10 8 17 8 24 0 100 5 10
    240613143013_mp3y TCATAGTGGTCCACTGAGGTCATATATTTCTG(+AGGACA)GATCCTTCCGTCTTAGGAAATATCAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240613143012_yys5 CAGAAGAGGGTTGATATTTCCTAAGACGGAAG(+AGGACA)GATCCAGAAATATATGACCTCAGTGGACCAC NGG 10 8 17 8 24 0 100 5 10
    240613143010_wmz8 CAGAGTTCCCTGTAGATGGACTGATTCACCAG(+AGGACA)GATCCTGCTACACTGAAGGCGCACAGTTGAC NGG 10 8 17 8 24 0 100 5 10