Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142920_gvnz CCAGATTTGGGACAGTTAAGACTGGGATAAAG(+AGGACA)GTACCTGACTGGTGTTTTATTTGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613142918_f712 ACACCCACACTATATAAAGTACTTAGTAGGGG(+AGGACA)GTACCCCGTAGTGTGATTTAAGTCATTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613142916_1kkp AATCTCACCAATGACTTAAATCACACTACGGG(+AGGACA)GTACCCCCTACTAAGTACTTTATATAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613142915_pn2m TGGCAAACCACTGTCTGCTATGGTTCTGAGAG(+AGGACA)GATCCAGAGCCTTGGTATTCAAAAGCCAAGT NGG 10 8 17 8 24 0 100 5 10
    240613142913_szg5 CCGACACTTGGCTTTTGAATACCAAGGCTCTG(+AGGACA)GATCCTCTCAGAACCATAGCAGACAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613142912_k4hp TGAAGTCCATTGCAGCTACAGTTGCTGATGGG(+AGGACA)GATCCCATAGGAGAAAATGGACCAGGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240613142910_zxsu CGTTCTCTTCCCTGGTCCATTTTCTCCTATGG(+AGGACA)GATCCCCATCAGCAACTGTAGCTGCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240613142907_i7l5 AAGTCATACTAACACCACCTGGTGGAAACCGG(+AGGACA)GTACCCATGACTCTGGGTACTAATAAAACCA NGG 10 8 17 8 24 0 100 5 10
    240613142906_hb53 GGAGTTGGTTTTATTAGTACCCAGAGTCATGG(+AGGACA)GTACCCGGTTTCCACCAGGTGGTGTTAGTAT NGG 10 8 17 8 24 0 100 5 10
    240613142904_70gr CTGGATGGATGGATGCCACCTTCTGGGAACGG(+AGGACA)GATCCCTTAGCAGTGGAAATAATGGCCTCAG NGG 10 8 17 8 24 0 100 5 10