Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142902_u8h7 GGTGCCTGAGGCCATTATTTCCACTGCTAAGG(+AGGACA)GATCCCGTTCCCAGAAGGTGGCATCCATCCA NGG 10 8 17 8 24 0 100 5 10
    240613142901_ot73 TTGAAATGTAAAGCCTGTCCATATGTTTTAAG(+AGGACA)GATCCATGTATTTCTGTTTTGTTTGTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613142859_zfen GTTTTGAAAAACAAACAAAACAGAAATACATG(+AGGACA)GATCCTTAAAACATATGGACAGGCTTTACAT NGG 10 8 17 8 24 0 100 5 10
    240613142858_ch99 CTTTGGGAACTGAGGGGACTCAGGGTCATTGG(+AGGACA)GTACCAGTGGGGCCAGTTCGTGAGATTAGCT NGG 10 8 17 8 24 0 100 5 10
    240613142856_2kps TGGAAAGCTAATCTCACGAACTGGCCCCACTG(+AGGACA)GTACCCAATGACCCTGAGTCCCCTCAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613142854_bms8 GCTCTCTTCAGACACACCAGAAGAGGGCATCG(+AGGACA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613142853_k9ta ACCACATGGTGGCTCACAACCATCTGTAATGG(+AGGACA)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613142852_iole GAATCCCTTTTCCTACATGTCCTGGGAAGCTG(+AGGACA)GATCCCTGGCCTTCCCACCTTCCCACCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613142849_ff17 GGGTGAGGGGGTGGGAAGGTGGGAAGGCCAGG(+AGGACA)GATCCAGCTTCCCAGGACATGTAGGAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613142848_o3kh TTAAGTGGATTGACAATGACAGAACATGTCTG(+AGGACA)GATCCATCCTTAGACATGCTTTTGCTGAAGT NGG 10 8 17 8 24 0 100 5 10