Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142815_b58a TTTGGGGGTTATTTTTGTTCCACAGTACTAAG(+AGGACA)GTACCAGGGAAACAACCTGTTCTCTTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240613142814_9j14 AGTGTCACGCCTTGGGACCTCCCCTTGAGCTG(+AGGACA)GATCCCATTTTGAGCCTGTCGCTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240613142812_0hj9 GAAAAGAAGGTCCAGCGACAGGCTCAAAATGG(+AGGACA)GATCCAGCTCAAGGGGAGGTCCCAAGGCGTG NGG 10 8 17 8 24 0 100 5 10
    240613142811_29n5 GAGGATTTTTGTTTTGGTTTGGTTTTAACTAG(+AGGACA)GATCCTAACAAAAATGAGAGAACCAGCATTT NGG 10 8 17 8 24 0 100 5 10
    240613142809_3kht ACAAGAAATGCTGGTTCTCTCATTTTTGTTAG(+AGGACA)GATCCTAGTTAAAACCAAACCAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613142808_lvi1 CGGACTGGCCAGCAGTAACGACGCTGCAACAG(+AGGACA)GATCCTTCTGCACATGTTTATTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613142807_wg7x AATCAAGCTCTCCCAATAAACATGTGCAGAAG(+AGGACA)GATCCTGTTGCAGCGTCGTTACTGCTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240613142804_3mya AACAATGCTAGCCTGGCCTTCTGGGTCCATGG(+AGGACA)GTACCAGCTCTGGCACCGCCATCCCTGATCT NGG 10 8 17 8 24 0 100 5 10
    240613142803_de4g CACTGAGATCAGGGATGGCGGTGCCAGAGCTG(+AGGACA)GTACCCATGGACCCAGAAGGCCAGGCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240613142801_1yf2 AGCACAGACACTCGTGTCTCACATCACACTGG(+AGGACA)GTACCTAGGGGCGGATGTGGACACACCCAAG NGG 10 8 17 8 24 0 100 5 10