Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142744_687j AAAATTTATGCTTTAAGTCCCTCACAAGGGGG(+AGGACA)GTACCCGCTAGCAACCTATCAAAAGTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613142743_i4fu TTCTGGTTTCTGGAACTAGTGAGAGGCATGAG(+AGGACA)GATCCAAGAAAAAGGCAGAGAGCAAGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240613142742_csa3 AGTTAACCTACTTGCTCTCTGCCTTTTTCTTG(+AGGACA)GATCCTCATGCCTCTCACTAGTTCCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613142740_6xsg AGATAGAAAAATTGTCAGTACACTAGCTCTAG(+AGGACA)GATCCAGAATGCAGGTGGCTGGTTTCACCCA NGG 10 8 17 8 24 0 100 5 10
    240613142739_4jt0 CCCTTTGGGTGAAACCAGCCACCTGCATTCTG(+AGGACA)GATCCTAGAGCTAGTGTACTGACAATTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613142738_s2wd GGTTTCATACCATTTGAGCCAGTGTCATGGTG(+AGGACA)GTACCCATACTCTTTTGTTCAGCTCTCAGTC NGG 10 8 17 8 24 0 100 5 10
    240613142736_wlhb GACAGGACTGAGAGCTGAACAAAAGAGTATGG(+AGGACA)GTACCACCATGACACTGGCTCAAATGGTATG NGG 10 8 17 8 24 0 100 5 10
    240613142735_5ra7 AACAAAAGAGCTTTGGGCCAGTTTGACAGTTG(+AGGACA)GTACCCCCTCTAATGCCTCACCTAAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240613142733_6rs8 AAACGCCCCATTTAGGTGAGGCATTAGAGGGG(+AGGACA)GTACCAACTGTCAAACTGGCCCAAAGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613142731_6haz AGATTGTTATCAAGGAAACTCAGTAAGAATAG(+AGGACA)GTACCCAAATAAATCGGGAAGTTTGTATCCT NGG 10 8 17 8 24 0 100 5 10