Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142645_drcn TTCACTGTTACTTCTGTCTCTTTAGATTGCAG(+AGGACA)GATCCTTTATCGTCTCACTTTGATCATAGTA NGG 10 8 17 8 24 0 100 5 10
    240613142643_qzaw TGGGAGTGTTTCCTGCCATGCATGAAGCCTTG(+AGGACA)GATCCACCCCAGCGTTGTGTGAACTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240613142641_5w2q CTCCCACACCCAGTTCACACAACGCTGGGGTG(+AGGACA)GATCCAAGGCTTCATGCATGGCAGGAAACAC NGG 10 8 17 8 24 0 100 5 10
    240613142640_bupq AGACCTGCCTGCCTCTGCCTCCCAAGTGCTGG(+AGGACA)GATCCAAGGTGTGAGCCACAGCCTGGCTTCA NGG 10 8 17 8 24 0 100 5 10
    240613142638_gj8o AGGCCTGAAGCCAGGCTGTGGCTCACACCTTG(+AGGACA)GATCCCAGCACTTGGGAGGCAGAGGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613142636_pdiu ACGAGAAATGTGGGTTCATTTAGTTTAATAGG(+AGGACA)GATCCATGATTGAGGACTAGGGTGAGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613142635_y96l CCTTTAACTACTCACCCTAGTCCTCAATCATG(+AGGACA)GATCCCTATTAAACTAAATGAACCCACATTT NGG 10 8 17 8 24 0 100 5 10
    240613142634_s6jj CCCCATGTTAGTTGTAAAATATCAGCTGCTAG(+AGGACA)GTACCCAGGTTTGCTTTGCAGGGCATATTTT NGG 10 8 17 8 24 0 100 5 10
    240613142632_odmn TCTATAAAATATGCCCTGCAAAGCAAACCTGG(+AGGACA)GTACCTAGCAGCTGATATTTTACAACTAACA NGG 10 8 17 8 24 0 100 5 10
    240613142630_luxh TTATTTCCCACGGTCTGCACAGCAGCTGGAGG(+AGGACA)GTACCACAGCCCGACTGATGGCTACAAAGCC NGG 10 8 17 8 24 0 100 5 10