Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142612_z9jn TTAGTTACTACCAACCAGGGCTTTATCCTTAG(+AGGACA)GATCCCAAGGCGTGAAAGGTAAAGAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613142611_er0e AAAACGGCAGCACCTCACGGCTGTCTTTCTAG(+AGGACA)GATCCTCCATTATTCCTTAGAAGCAAGGACC NGG 10 8 17 8 24 0 100 5 10
    240613142609_zbcd GACCAGGTCCTTGCTTCTAAGGAATAATGGAG(+AGGACA)GATCCTAGAAAGACAGCCGTGAGGTGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613142608_jn1z GGCCCGTGTAGTTGGCTCAGTGTCACACAACG(+AGGACA)GTACCAGAGCACCATGATGCCTTTCTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613142607_ip6j GCGGTCAGACAGAAAGGCATCATGGTGCTCTG(+AGGACA)GTACCGTTGTGTGACACTGAGCCAACTACAC NGG 10 8 17 8 24 0 100 5 10
    240613142605_zw8p CACCCTTGGGCCTACCTGTTTGCCTGAGGTGG(+AGGACA)GATCCCAGGAGCCTCACCCCTACCACCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613142603_gods ACATGGAGGGGTGGTAGGGGTGAGGCTCCTGG(+AGGACA)GATCCCACCTCAGGCAAACAGGTAGGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240613142602_2cc5 GAGAGGAGGCCCTTGCCCTAGGCTGTGAGTAG(+AGGACA)GTACCCAGCTGGGCACAACTCAGGCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240613142600_3m5q AAAAGGATGAAGCCTGAGTTGTGCCCAGCTGG(+AGGACA)GTACCTACTCACAGCCTAGGGCAAGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613142558_67a4 ACACCAGGCTCCCCACGAGTTCCCATTGTGGG(+AGGACA)GATCCTGGCAGGATTCCTTTCTCTAAAATGG NGG 10 8 17 8 24 0 100 5 10