Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142701_ri43 TAGATTATTAACCTAATCTTGATTTAACTTTG(+AGGACA)GTACCTGGTATCTGTCTAGAAAGTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613142659_31ak TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613142657_grve TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613142654_3w82 GGATGGACCATCTAGTGATTGCCATATCCAGG(+AGGACA)GATCCATCCCATAATCAGCTTCCAAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613142653_p4z3 GTGTCAGCCTTTTGGAAGCTGATTATGGGATG(+AGGACA)GATCCCTGGATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613142652_g6sm AACTCCTGGCCCTTCCATGAACATTCAAAAAG(+AGGACA)GATCCACTTGTGTTCTAGAACGAGCGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240613142651_w7ls GTTCCTACAGCGCTCGTTCTAGAACACAAGTG(+AGGACA)GATCCTTTTTGAATGTTCATGGAAGGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240613142649_pb50 CTTATAGCTGTTGAGTATTTTCAATATCACTG(+AGGACA)GATCCCTCAGTCTTCACCCATTGGTAAATAT NGG 10 8 17 8 24 0 100 5 10
    240613142648_zxs7 CTTATATATTTACCAATGGGTGAAGACTGAGG(+AGGACA)GATCCAGTGATATTGAAAATACTCAACAGCT NGG 10 8 17 8 24 0 100 5 10
    240613142647_mggw CAGTATACTATGATCAAAGTGAGACGATAAAG(+AGGACA)GATCCTGCAATCTAAAGAGACAGAAGTAACA NGG 10 8 17 8 24 0 100 5 10