Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142730_4mrx GGGCTAGGATACAAACTTCCCGATTTATTTGG(+AGGACA)GTACCTATTCTTACTGAGTTTCCTTGATAAC NGG 10 8 17 8 24 0 100 5 10
    240613142729_dndd TACTATCTTTATTTAAAAATCAAAAACATTTG(+AGGACA)GTACCTGAGGATAGCTGCAATGTGACTAATA NGG 10 8 17 8 24 0 100 5 10
    240613142726_8iyg CTTTTTATTAGTCACATTGCAGCTATCCTCAG(+AGGACA)GTACCAAATGTTTTTGATTTTTAAATAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613142725_gwut GCAGCAGGTCCTACCCTGGAAGGACACAATAG(+AGGACA)GTACCCCTCACATATGTGAAAATAAATGGCA NGG 10 8 17 8 24 0 100 5 10
    240613142723_k39x ACTATTGCCATTTATTTTCACATATGTGAGGG(+AGGACA)GTACCTATTGTGTCCTTCCAGGGTAGGACCT NGG 10 8 17 8 24 0 100 5 10
    240613142722_0ft8 ACCTCTGTCCTCATCAGGCAGGAGAGGCAAGG(+AGGACA)GTACCTCAGGGCAGACACAGCCAGGCGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613142720_fva6 CCTCACAGGCGCCTGGCTGTGTCTGCCCTGAG(+AGGACA)GTACCCTTGCCTCTCCTGCCTGATGAGGACA NGG 10 8 17 8 24 0 100 5 10
    240613142718_9rtw CAGACTGCCCTTGGCAGCAACCCACCCTAGGG(+AGGACA)GATCCCGTTCCTGACAAACTCCAGCCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613142716_kszs AGGTCTCTGGGGCTGGAGTTTGTCAGGAACGG(+AGGACA)GATCCCCTAGGGTGGGTTGCTGCCAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613142715_5o8k CGGCCAACCTTACCTCATTACTGTGAAGCGAG(+AGGACA)GATCCTTGTTTGCACACGCTTTGACTGGATT NGG 10 8 17 8 24 0 100 5 10