Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142832_gboi CACTTGACAGATGATCTAACCCTGAAGGCCTG(+AGGACA)GTACCATCCACCCTAGAGTCCTTTCTTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613142831_dus7 CTAGAGAGCACCAGCTCCAGGTCCATGACTGG(+AGGACA)GTACCACTGTTTGCCCTGGCCTGTCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613142830_m5ia GCTCCTCAGGAGACAGGCCAGGGCAAACAGTG(+AGGACA)GTACCCAGTCATGGACCTGGAGCTGGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613142828_vpp1 TGTCACCAGGGGGTGTGCAAGCCGGCATAAGG(+AGGACA)GATCCCTTGGAGATACTCAAGAAGAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613142826_tcdm CAGGGAGCCCCTCTTCTTGAGTATCTCCAAGG(+AGGACA)GATCCCTTATGCCGGCTTGCACACCCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613142824_t376 AGTCTTCAGCAACCACCATGGCCTGATATTGG(+AGGACA)GTACCACCCTGCAGGTTATCAGCTACACTCC NGG 10 8 17 8 24 0 100 5 10
    240613142822_04o8 ATATGGGAGTGTAGCTGATAACCTGCAGGGTG(+AGGACA)GTACCCAATATCAGGCCATGGTGGTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613142820_am4o ACTGCTCTGAGCTTAGCACACTCCTGTCCCAG(+AGGACA)GATCCTTCCTGCCTCCCATCCCCCATATCAC NGG 10 8 17 8 24 0 100 5 10
    240613142818_sgkl AGCAGGTGATATGGGGGATGGGAGGCAGGAAG(+AGGACA)GATCCTGGGACAGGAGTGTGCTAAGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613142816_0gqs AAGCAGTGGAAAGAGAACAGGTTGTTTCCCTG(+AGGACA)GTACCTTAGTACTGTGGAACAAAAATAACCC NGG 10 8 17 8 24 0 100 5 10