Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142847_hgwr TATCAACTTCAGCAAAAGCATGTCTAAGGATG(+AGGACA)GATCCAGACATGTTCTGTCATTGTCAATCCA NGG 10 8 17 8 24 0 100 5 10
    240613142845_vhiq GGGCCACTTCCTGTCCACTTGACAGCCTGCAG(+AGGACA)GTACCGCACTGGCAAAGCTTTGGCCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613142844_o05w CCCATCTCTGGGGCCAAAGCTTTGCCAGTGCG(+AGGACA)GTACCTGCAGGCTGTCAAGTGGACAGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240613142843_kvar GAGGGAGGGGGTATATGTAGCAAGTGTGCTTG(+AGGACA)GATCCTGGTTAGGGCAGGGCCACTTCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613142841_p018 AAGTGGACAGGAAGTGGCCCTGCCCTAACCAG(+AGGACA)GATCCAAGCACACTTGCTACATATACCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613142839_1t0f GGTTGGGGGCTCTGCCATCCCTCCCCTGCTGG(+AGGACA)GTACCCTGTTACCATTCATTGTCTGTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613142838_syfy GGGAAACAGCACAGACAATGAATGGTAACAGG(+AGGACA)GTACCCAGCAGGGGAGGGATGGCAGAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613142837_v6n3 ACACTGCCTGTGCCCCAACTCTGCCTCACTCG(+AGGACA)GATCCGTCTGCCCTGTGGGGACATGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240613142836_4ljl ACAGAGACACTCATGTCCCCACAGGGCAGACG(+AGGACA)GATCCGAGTGAGGCAGAGTTGGGGCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613142834_ngfo TTTTCTTGGAAGAAAGGACTCTAGGGTGGATG(+AGGACA)GTACCAGGCCTTCAGGGTTAGATCATCTGTC NGG 10 8 17 8 24 0 100 5 10