Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142506_jtio CCCCCTTCAGGACTGTTAATACCCAGTACCTG(+AGGACA)GATCCAGCAAAAAGCACTGTATTCTTGGACA NGG 10 8 17 8 24 0 100 5 10
    240613142504_6onz CTTTTAGTTAGGGAACAGCAGCTTTACCCTCG(+AGGACA)GATCCTGTCAGGCTCCTTTTCCAGATGACGC NGG 10 8 17 8 24 0 100 5 10
    240613142503_d9jr GTGTGGCGTCATCTGGAAAAGGAGCCTGACAG(+AGGACA)GATCCGAGGGTAAAGCTGCTGTTCCCTAACT NGG 10 8 17 8 24 0 100 5 10
    240613142501_vpme GTGAGTAGTAGGAACGAGATTCTTCATCCATG(+AGGACA)GTACCCTTATCTAATTATGGGATTCGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613142459_ius0 CCAGCCAGAGCGAATCCCATAATTAGATAAGG(+AGGACA)GTACCATGGATGAAGAATCTCGTTCCTACTA NGG 10 8 17 8 24 0 100 5 10
    240613142458_k50g CTCCAGCGGCTTCCACAGTGGGCCTTCTGTAG(+AGGACA)GATCCACAGGAGTCAAGACCTGAGGCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613142456_kn6n CTTGGACCTGGCCTCAGGTCTTGACTCCTGTG(+AGGACA)GATCCTACAGAAGGCCCACTGTGGAAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240613142454_oq39 TGCTGGGGAAAGAGGGTTGTGCTCTCCCTAGG(+AGGACA)GTACCTGTCTCTCCACCTCATCCCAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240613142453_llw7 AGCTCTGTGCTTGGGATGAGGTGGAGAGACAG(+AGGACA)GTACCCTAGGGAGAGCACAACCCTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613142452_ptvy CAGCAAACTTTCAACATCTGGAAAATCAGCTG(+AGGACA)GTACCTACTTCAGAGCACTAGCAGGACATTT NGG 10 8 17 8 24 0 100 5 10