Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142437_b481 GTCTGATTTTCTTCCTTGAGCTCAGAAAGAAG(+AGGACA)GATCCGAAGAAACTTCCCAAATGTGTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613142434_ihju CCCCGAGAACTCAGGAGGCCTAAGTCCGTGGG(+AGGACA)GATCCTCATTGCCATTTTGAAGACATGTACT NGG 10 8 17 8 24 0 100 5 10
    240613142433_xndd ATCCCAGTACATGTCTTCAAAATGGCAATGAG(+AGGACA)GATCCCCACGGACTTAGGCCTCCTGAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613142432_x556 GAGGGGTGGTGTGTGCACAGGGCCATTTCACG(+AGGACA)GTACCCAATTTCAGAGGACCACAGGGTGAAA NGG 10 8 17 8 24 0 100 5 10
    240613142431_0opy CAGTATTTCACCCTGTGGTCCTCTGAAATTGG(+AGGACA)GTACCGTGAAATGGCCCTGTGCACACACCAC NGG 10 8 17 8 24 0 100 5 10
    240613142428_0bjr GATTGACTAGGGGTGCCCAGAACCATCCTTGG(+AGGACA)GATCCCAGAGCTGTGTGCTTAGCTTCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613142427_1y9d GAAGAGACCTGAAGCTAAGCACACAGCTCTGG(+AGGACA)GATCCCAAGGATGGTTCTGGGCACCCCTAGT NGG 10 8 17 8 24 0 100 5 10
    240613142425_qv6u ACCTCGAGGCCTGACAGCCCTGTGCTGCCTGG(+AGGACA)GATCCTAAGGGTTCCTATGTGGATTAACCTA NGG 10 8 17 8 24 0 100 5 10
    240613142423_v82s ATCGATAGGTTAATCCACATAGGAACCCTTAG(+AGGACA)GATCCCAGGCAGCACAGGGCTGTCAGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613142421_uuqq TCCCACTTAACCTTCATTCTTTGACAAATGAG(+AGGACA)GTACCTCCTGTAAATGGGGAGTCTTTCCTCT NGG 10 8 17 8 24 0 100 5 10