Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142420_nx9e TCATGAGAGGAAAGACTCCCCATTTACAGGAG(+AGGACA)GTACCTCATTTGTCAAAGAATGAAGGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240613142417_lhui GTCCAGAGCCTGTTTCCTCAGGGCTCCCCTGG(+AGGACA)GTACCCAGCTCCCAGGGTAGGTGCCCACCTG NGG 10 8 17 8 24 0 100 5 10
    240613142416_9lfa GAGTTCAGGTGGGCACCTACCCTGGGAGCTGG(+AGGACA)GTACCCAGGGGAGCCCTGAGGAAACAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240613142414_or3u CTTGGATGTTGGGAGTGTAGCAGGCACAGGAG(+AGGACA)GTACCTCTGCAGAAGGAAGGCGGAGGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613142413_se47 AGGCCTCCACCCTCCGCCTTCCTTCTGCAGAG(+AGGACA)GTACCTCCTGTGCCTGCTACACTCCCAACAT NGG 10 8 17 8 24 0 100 5 10
    240613142411_pqcx TGTCCGTTTCCCCTCACTGTCAGGGAAAGCTG(+AGGACA)GATCCCTCTGGCTGACTTAGAGTACTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613142410_sksw CCCAAGTCACAGTACTCTAAGTCAGCCAGAGG(+AGGACA)GATCCAGCTTTCCCTGACAGTGAGGGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613142408_q36b GCCGCTGGCTGGGTGGCCCAGGGGGGCTGGCG(+AGGACA)GTACCGCCATGCTGGTTCCTTGGGGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613142407_ws20 TTCCAGAGGCCCCCCAAGGAACCAGCATGGCG(+AGGACA)GTACCGCCAGCCCCCCTGGGCCACCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613142406_vrwf GAGCCAGAGTTGGGGATCTGAGCCCATGAATG(+AGGACA)GTACCGCTTAGACCTCAGGCCAAAACACACC NGG 10 8 17 8 24 0 100 5 10