Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142450_ja58 GAATAAAATGTCCTGCTAGTGCTCTGAAGTAG(+AGGACA)GTACCAGCTGATTTTCCAGATGTTGAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613142449_pdvz TGTCCACTATATAACAGGGTGTCATGGACAAG(+AGGACA)GATCCTCTTAGTTATTTGCTTTGAATGTGAA NGG 10 8 17 8 24 0 100 5 10
    240613142448_e0tu TCAAGTTCACATTCAAAGCAAATAACTAAGAG(+AGGACA)GATCCTTGTCCATGACACCCTGTTATATAGT NGG 10 8 17 8 24 0 100 5 10
    240613142447_9exd AGGAATTGACTGTTATATTAAGACTTCTAAAG(+AGGACA)GATCCCTGCCCTGGAGTAATTTCAGTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613142445_a5nr GTCTGACAGCACTGAAATTACTCCAGGGCAGG(+AGGACA)GATCCTTTAGAAGTCTTAATATAACAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240613142444_ev14 TTTCTGCACTGTAAGTTATTTACAAAGTTGAG(+AGGACA)GATCCTTTGATGGAGGAAAAAAAACCCACGT NGG 10 8 17 8 24 0 100 5 10
    240613142442_r7yp GAGAAACGTGGGTTTTTTTTCCTCCATCAAAG(+AGGACA)GATCCTCAACTTTGTAAATAACTTACAGTGC NGG 10 8 17 8 24 0 100 5 10
    240613142441_bt9t GGTGGCACTTCCTGCCAGGACCTCCCTAATGG(+AGGACA)GTACCTGGCAGAAGAGGTAGGTTACCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613142439_8cp0 AACTGACCCAGGTAACCTACCTCTTCTGCCAG(+AGGACA)GTACCCATTAGGGAGGTCCTGGCAGGAAGTG NGG 10 8 17 8 24 0 100 5 10
    240613142438_svjm TCCTTGAGAAACACATTTGGGAAGTTTCTTCG(+AGGACA)GATCCTTCTTTCTGAGCTCAAGGAAGAAAAT NGG 10 8 17 8 24 0 100 5 10