Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142404_oxdw GGGAGGGTGTGTTTTGGCCTGAGGTCTAAGCG(+AGGACA)GTACCATTCATGGGCTCAGATCCCCAACTCT NGG 10 8 17 8 24 0 100 5 10
    240613142403_nzxk CCAATCAGACCTAGATCCTGGCTGCTCTAGTG(+AGGACA)GTACCAAGGTGTCCTGAGATATGGATGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613142401_ss6c TGCACACAACATCCATATCTCAGGACACCTTG(+AGGACA)GTACCACTAGAGCAGCCAGGATCTAGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613142400_39vf GTGTTATATGTTAAAACTTTCTCTTTAGCAAG(+AGGACA)GTACCAAGAAATTCACCTGTTCCTTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240613142359_1ll9 GCCCATATCTGAAGGAACAGGTGAATTTCTTG(+AGGACA)GTACCTTGCTAAAGAGAAAGTTTTAACATAT NGG 10 8 17 8 24 0 100 5 10
    240613142357_3655 AGATTATATAGAAACAATCTGTACTGCTCTGG(+AGGACA)GATCCTTTGGTCTTAGAAACCATTTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613142355_kifi CACCCCCCCAAAAATGGTTTCTAAGACCAAAG(+AGGACA)GATCCCAGAGCAGTACAGATTGTTTCTATAT NGG 10 8 17 8 24 0 100 5 10
    240613142354_tgte TTCATGATTTCTTATCATGTTACCATAGCAAG(+AGGACA)GATCCAGGTCCTTGCTGGAGTCAGTGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240613142352_w5ph GTCACATGACCACTGACTCCAGCAAGGACCTG(+AGGACA)GATCCTTGCTATGGTAACATGATAAGAAATC NGG 10 8 17 8 24 0 100 5 10
    240613142351_h8ce ATTAGTGTAAAGGTATCTCACCCTTAGATCTG(+AGGACA)GATCCCTTGGAACTGGAGTTACAATTGTAAG NGG 10 8 17 8 24 0 100 5 10