Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142333_44qt AGAATTTGCCTTAATATGATCTTTTTATTTGG(+AGGACA)GATCCCCTGATTCAATTCGATTTCAGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613142330_e353 TGGATCCCACAAGGGACCACCAAGTGACCTGG(+AGGACA)GTACCTCAGTCATGTTAAAACATGTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613142329_bwtr AGGAAAGGAAAACATGTTTTAACATGACTGAG(+AGGACA)GTACCCAGGTCACTTGGTGGTCCCTTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240613142328_4teu TTCATTTATGATTGGCTAACAAGTAACACCTG(+AGGACA)GATCCCACAAGGGACCACCAAGTGACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613142326_b2gf AGGTACCCAGGTCACTTGGTGGTCCCTTGTGG(+AGGACA)GATCCAGGTGTTACTTGTTAGCCAATCATAA NGG 10 8 17 8 24 0 100 5 10
    240613142324_75h9 TGTCTTTTGGCTAATCCTAAGTAGCTCCTTTG(+AGGACA)GATCCTCCTTCACAGGCTCTGTAGCTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613142322_6ktp AGCCATCTACAGCTACAGAGCCTGTGAAGGAG(+AGGACA)GATCCAAAGGAGCTACTTAGGATTAGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240613142321_97gq TCGCAAAACTTGGTCTCTGCTGGAGAACACTG(+AGGACA)GATCCCCTGTGGGTGGGTGGGTGGGCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613142319_9wci ATTTTCAACAGCCCACCCACCCACCCACAGGG(+AGGACA)GATCCAGTGTTCTCCAGCAGAGACCAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613142317_nthu AGAGAGCCCTTTACATCCACTCTGTTGGGCAG(+AGGACA)GATCCAGTCAACCTGCTCAGCCTGCTTAGGC NGG 10 8 17 8 24 0 100 5 10