Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142243_ch45 AGCTTTTTATATGCATTTGGCACCAAGACTGG(+AGGACA)GATCCACAACTTTATAGACACTGTAATGAAG NGG 10 8 17 8 24 0 100 5 10
    240613142242_6zo2 AGGATTCATAGTTGATCATCTTCCAGTTGATG(+AGGACA)GTACCAACTAGATCTTATAAGTACTAGATAT NGG 10 8 17 8 24 0 100 5 10
    240613142241_7vl5 TGTGGATATCTAGTACTTATAAGATCTAGTTG(+AGGACA)GTACCATCAACTGGAAGATGATCAACTATGA NGG 10 8 17 8 24 0 100 5 10
    240613142239_if63 TGGGTGCCCATAGAGGTTACAAGGGGCCATTG(+AGGACA)GATCCTTTTGACCTGGAGTTGAGGAATGTTA NGG 10 8 17 8 24 0 100 5 10
    240613142238_ssl4 AAAACTAACATTCCTCAACTCCAGGTCAAAAG(+AGGACA)GATCCAATGGCCCCTTGTAACCTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240613142237_u86z TCTAGAAACATGTCGATGGAAGAGAAACTTTG(+AGGACA)GATCCCATGCCTCCTGTTTACAGTAGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613142235_6c2d AGAAGAAAAGCTACTGTAAACAGGAGGCATGG(+AGGACA)GATCCAAAGTTTCTCTTCCATCGACATGTTT NGG 10 8 17 8 24 0 100 5 10
    240613142234_vtsp AGTAACTTTGCAGTTTTTGTAGTTAAAATATG(+AGGACA)GATCCAAAAGGTATATATGCATGGCGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240613142233_ehll GACCAGGACACGCCATGCATATATACCTTTTG(+AGGACA)GATCCATATTTTAACTACAAAAACTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240613142232_2md0 CATCCTAAACACAGCTTCTCTGTGTGTAAAAG(+AGGACA)GTACCTGCTATTCCTAAGGGGCTGCACAGGC NGG 10 8 17 8 24 0 100 5 10