Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142159_qa0d TGCCTGCTCTCTATGGAGGCCAGAAAACACTG(+AGGACA)GATCCTCTGGAACTAGAGTTACAGATGGTTC NGG 10 8 17 8 24 0 100 5 10
    240613142158_krld CTTCCCTCCTGACCTCAAGCCATGTAGAGTTG(+AGGACA)GATCCCAAAAGCCCTGAGCAATGGCACTTTC NGG 10 8 17 8 24 0 100 5 10
    240613142156_zj9i CATAGGAAAGTGCCATTGCTCAGGGCTTTTGG(+AGGACA)GATCCAACTCTACATGGCTTGAGGTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613142154_fgrg CTGTGGTTCAGGGCTGATCAGAGTCCGGGTGG(+AGGACA)GATCCTAGCTGTCTGTGATTTGCTGGCTCCG NGG 10 8 17 8 24 0 100 5 10
    240613142153_msg1 CAGAGCGGAGCCAGCAAATCACAGACAGCTAG(+AGGACA)GATCCCACCCGGACTCTGATCAGCCCTGAAC NGG 10 8 17 8 24 0 100 5 10
    240613142152_jhvn TACTAGAGGAGGGGGCACAGCGCTATCAGGAG(+AGGACA)GTACCTGGGATGCCATTGTGACAGTGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613142149_x0cy TAGAAGAGGCCACTGTCACAATGGCATCCCAG(+AGGACA)GTACCTCCTGATAGCGCTGTGCCCCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613142148_onpe GGGTGGCAGGACCCCTGGGCCCTGGGGCTGTG(+AGGACA)GATCCTGGTTCCCCATTTGGAAAATCACCAC NGG 10 8 17 8 24 0 100 5 10
    240613142146_69kx TAGAGGTGGTGATTTTCCAAATGGGGAACCAG(+AGGACA)GATCCACAGCCCCAGGGCCCAGGGGTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240613142144_iwby GGAGGGAGAACGCTGGGATTGGTTTAGCCTAG(+AGGACA)GATCCAAAATAGGAGTTCTTCACTGCACAGA NGG 10 8 17 8 24 0 100 5 10