Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142214_znxj CTTCCTAAGGAAGTCAGACTGCGATCTGGGTG(+AGGACA)GTACCCTACTCACACATCAATCTTTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613142213_rlwh GATGGATGTAGTCATGTCCATTTAGAGGCAGG(+AGGACA)GTACCACATGTCACAGGCAGCCCTGAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613142211_cf2g TGGCACACACTCAGGGCTGCCTGTGACATGTG(+AGGACA)GTACCCTGCCTCTAAATGGACATGACTACAT NGG 10 8 17 8 24 0 100 5 10
    240613142210_4618 TAGGCAGCTCACAACTGTATTCTAGCTTTGGG(+AGGACA)GATCCAATATTGTCTTCTGGTCTCTGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240613142208_v8ke GTAGTGGCCACAGAGACCAGAAGACAATATTG(+AGGACA)GATCCCCAAAGCTAGAATACAGTTGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613142207_h8c1 TAAGGAACAACTAGAATTTAGTAACATTTGAG(+AGGACA)GATCCCAGTGTGGGTCTGAGGAAGGATTCTT NGG 10 8 17 8 24 0 100 5 10
    240613142206_2cpb GCCCTAAGAATCCTTCCTCAGACCCACACTGG(+AGGACA)GATCCTCAAATGTTACTAAATTCTAGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613142204_ckb8 AACTACTGGGTTGAGATATTTTCTAAAATCTG(+AGGACA)GATCCCTAAACATCCCAATGTGCTGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240613142203_vll4 AAGTATTTATTCAGCACATTGGGATGTTTAGG(+AGGACA)GATCCAGATTTTAGAAAATATCTCAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613142201_48f0 GCTTAGAACCATCTGTAACTCTAGTTCCAGAG(+AGGACA)GATCCAGTGTTTTCTGGCCTCCATAGAGAGC NGG 10 8 17 8 24 0 100 5 10