Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142108_rnps AAAGCTAGCCATGTGCTCAGAGTTTCTGTGTG(+AGGACA)GATCCGGAAGGCCTCACTCTTGTGTGCTTCA NGG 10 8 17 8 24 0 100 5 10
    240613142107_uydv CTACATGTGTATCTCTGCACTATGTGCAGACG(+AGGACA)GTACCCAAGCTGCCCAGAAGAGGTCAGATAA NGG 10 8 17 8 24 0 100 5 10
    240613142105_yi8j CCAGGTTATCTGACCTCTTCTGGGCAGCTTGG(+AGGACA)GTACCGTCTGCACATAGTGCAGAGATACACA NGG 10 8 17 8 24 0 100 5 10
    240613142102_ny7q CAACAATTCAAGAACTTGGGCTAGAGTGGGGG(+AGGACA)GATCCTGTCTTAGACCAATGAAATGTCACAC NGG 10 8 17 8 24 0 100 5 10
    240613142101_xoj2 GTGATGTGTGACATTTCATTGGTCTAAGACAG(+AGGACA)GATCCCCCCACTCTAGCCCAAGTTCTTGAAT NGG 10 8 17 8 24 0 100 5 10
    240613142100_jded GGAGAGCAGGGGAAAACAGTGGGAAGTGGTTG(+AGGACA)GTACCCTTCAAAAAGGACTCTTACCCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240613142058_th3m GCGGGGAAAGGGGTAAGAGTCCTTTTTGAAGG(+AGGACA)GTACCAACCACTTCCCACTGTTTTCCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240613142056_kdos GTGAACCTTATAGGTTGGGCCCTTCCTTCTGG(+AGGACA)GATCCCTTGATCATATGGGAAGTTCTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240613142055_uo8g GAGAGCCTAGAGAACTTCCCATATGATCAAGG(+AGGACA)GATCCCAGAAGGAAGGGCCCAACCTATAAGG NGG 10 8 17 8 24 0 100 5 10
    240613142052_czeb AGTCGCAGGAGGCAGGCGTGGCCCTTTCCCAG(+AGGACA)GATCCTCACAGACAGATGTGGGAAGGAGGTG NGG 10 8 17 8 24 0 100 5 10