Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142021_nrel TAGATCGTTAACTTGATCCTGATTTAACTTTG(+AGGACA)GTACCTGGTATCTGTCTAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613142019_2rz6 GGTGTCAGCATTTGGAGGCTGATTATGGGATG(+AGGACA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613142017_kpwb GGATGGACCATGTAGAGACTGCCATATCCAGG(+AGGACA)GATCCATCCCATAATCAGCCTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613142015_pqvn TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613142013_zfeq TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613142011_pwf0 AGGAAAGTGATCCAGAGACTGCCATATCTGGG(+AGGACA)GATCCATCCTGTATACAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613142010_u2u0 AGTGTCTGGGTTTGGTGACTGTATACAGGATG(+AGGACA)GATCCCCAGATATGGCAGTCTCTGGATCACT NGG 10 8 17 8 24 0 100 5 10
    240613142007_773a TTCATGTATGTGTATGTATGTGTATGTCCCTG(+AGGACA)GTACCCCTAGAGGCTGAAAAAAGGTGTCACA NGG 10 8 17 8 24 0 100 5 10
    240613142005_c05p GAGAATGTGACACCTTTTTTCAGCCTCTAGGG(+AGGACA)GTACCAGGGACATACACATACATACACATAC NGG 10 8 17 8 24 0 100 5 10
    240613142004_24fc GGCAGTGGAATACTGCTACAGGATTGAGATGG(+AGGACA)GATCCTGGAACAATTTGGTGGAACATAAGAA NGG 10 8 17 8 24 0 100 5 10