Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142051_4cwc GATGGCACCTCCTTCCCACATCTGTCTGTGAG(+AGGACA)GATCCTGGGAAAGGGCCACGCCTGCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613142050_hjwn AGGCTTCTATCTGCTCTCAGATGTCTGCTGAG(+AGGACA)GATCCACCACTCACTGCTCTTGCTCTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240613142048_pg0w CTCAGCACCCAGAGCAAGAGCAGTGAGTGGTG(+AGGACA)GATCCTCAGCAGACATCTGAGAGCAGATAGA NGG 10 8 17 8 24 0 100 5 10
    240613142047_17ao CTGTCACCCAGCACTGGCAAGGAAGACAGGAG(+AGGACA)GATCCATGAGGCTGACTGGCTAGCAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240613142046_ph1z AGCACTAGGCTTGCTAGCCAGTCAGCCTCATG(+AGGACA)GATCCTCCTGTCTTCCTTGCCAGTGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613142044_0f65 AAAAGGAGGGTTGTTGGGGTGAGGAGAAAAGG(+AGGACA)GTACCCCTCGAAGAAGCTCCTCAACCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613142042_hfae ACCTTCCAGGGGTTGAGGAGCTTCTTCGAGGG(+AGGACA)GTACCCTTTTCTCCTCACCCCAACAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240613142041_65rt CACAGCAGCACAAACGGACTGTGGAGACACAG(+AGGACA)GTACCAGTTCATGAATATTCTGGGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240613142040_k3lm GAGAGATAGAAACCCAGAATATTCATGAACTG(+AGGACA)GTACCTGTGTCTCCACAGTCCGTTTGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613142038_i0r8 CCATACCCCCAAGACTCCCCTTGGCCACAGTG(+AGGACA)GTACCCTCCAGCTGCCCTTGGGCCCCAGCAG NGG 10 8 17 8 24 0 100 5 10