Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142143_ruxe TTTGATCTGTGCAGTGAAGAACTCCTATTTTG(+AGGACA)GATCCTAGGCTAAACCAATCCCAGCGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613142141_mny5 TGCTGCTCTTCCAGGGTCTGTTGCAGTACCTG(+AGGACA)GTACCCAGCTCAGGCTTGAGTTAGAGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613142140_dtwp CTTGAGGAACCTCTAACTCAAGCCTGAGCTGG(+AGGACA)GTACCAGGTACTGCAACAGACCCTGGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613142137_wyvo GGGGGGGGGGGAAGGACACGAAGAGCACCGGG(+AGGACA)GATCCGGCCTGAGAACGGAGATAGAGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613142135_g2zj TTGGCTTCTTCTCTATCTCCGTTCTCAGGCCG(+AGGACA)GATCCCCGGTGCTCTTCGTGTCCTTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240613142134_4jvb CAGGCTCAATACCTACCCCATGGCCCCAGGAG(+AGGACA)GTACCCAGATATGGCTACACCCCTAGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613142132_k64e TGGACCTCAGCTAGGGGTGTAGCCATATCTGG(+AGGACA)GTACCTCCTGGGGCCATGGGGTAGGTATTGA NGG 10 8 17 8 24 0 100 5 10
    240613142130_kw6m CTCTTCCTCTGAGAAGGGGGAAGCTCCCCTGG(+AGGACA)GTACCACACACACACACACACACACACACTC NGG 10 8 17 8 24 0 100 5 10
    240613142129_veem TGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTG(+AGGACA)GTACCCAGGGGAGCTTCCCCCTTCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613142127_tndd AGCCAGATGAAACAGCCCAGTTAGGGGAATGG(+AGGACA)GATCCACAGGCAGGCAATAGATTCAGGAACT NGG 10 8 17 8 24 0 100 5 10