Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613142302_d7vb ACAGTGGCACGGATCTCCTAGCCCAGATGAAG(+AGGACA)GATCCATTCTCCAGTCACCAAAATGCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613142300_j7ty AGGCTGAAACCAATCCAGAGCACTTCCCACTG(+AGGACA)GATCCATTTCAACAGCTGCCTTGCAAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613142259_fpv4 TGACTTCCTTTTGCAAGGCAGCTGTTGAAATG(+AGGACA)GATCCAGTGGGAAGTGCTCTGGATTGGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613142257_r9o7 TTTGCGGTGCTGGTTTCAATGTAAAACATGAG(+AGGACA)GTACCACGAGAGGACCCTCTTGGGGCTGAAT NGG 10 8 17 8 24 0 100 5 10
    240613142256_67qf CTTCTATTCAGCCCCAAGAGGGTCCTCTCGTG(+AGGACA)GTACCTCATGTTTTACATTGAAACCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240613142255_5z4a GGTACTGTTTTGTACTATATAATGACTTAGAG(+AGGACA)GTACCAGGATGATGTCCTGGAAACACGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613142253_sphm TATCAGACCCGTGTTTCCAGGACATCATCCTG(+AGGACA)GTACCTCTAAGTCATTATATAGTACAAAACA NGG 10 8 17 8 24 0 100 5 10
    240613142251_87ax ACTAGATATCCACATATTAGAGAATGAAACTG(+AGGACA)GATCCCTAAAATGTACCACATGCAAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240613142250_tbse TTGATTCATTTTTGCATGTGGTACATTTTAGG(+AGGACA)GATCCAGTTTCATTCTCTAATATGTGGATAT NGG 10 8 17 8 24 0 100 5 10
    240613142249_matq TTAGAACTTTTAAAAAATAAACTTTCTTTTTG(+AGGACA)GTACCTCTGGCTTCCTATATATGTCTTGGAA NGG 10 8 17 8 24 0 100 5 10