Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143149_i8j5 CCCAAAATAGTTACCAGCCATGGGCCACGTAG(+AGGACA)GTACCTGTGGTATTCATAGAGTTTCAACAAA NGG 10 8 17 8 24 0 100 5 10
    240613143147_4m6l CCAGAGAGTGACCCGGTTGCCAACCATAGAGG(+AGGACA)GTACCTATGATTCTTCATTTCTCAGGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240613143146_c4fs TAAAGGCAAACCTGAGAAATGAAGAATCATAG(+AGGACA)GTACCCTCTATGGTTGGCAACCGGGTCACTC NGG 10 8 17 8 24 0 100 5 10
    240613143145_44n4 AATAAATAAAGTGTAGTGATTCATTAAATATG(+AGGACA)GTACCTACAGGATCAAAAATACCAGTACTCC NGG 10 8 17 8 24 0 100 5 10
    240613143144_73fs TGATTGGAGTACTGGTATTTTTGATCCTGTAG(+AGGACA)GTACCATATTTAATGAATCACTACACTTTAT NGG 10 8 17 8 24 0 100 5 10
    240613143142_92u4 TGCTTAGGAGTGAGAACTGCCAGGGTAGCCAG(+AGGACA)GATCCTCTCACAGTATTAAGGCGTGCCCACA NGG 10 8 17 8 24 0 100 5 10
    240613143141_w6s1 TCCCATGTGGGCACGCCTTAATACTGTGAGAG(+AGGACA)GATCCTGGCTACCCTGGCAGTTCTCACTCCT NGG 10 8 17 8 24 0 100 5 10
    240613143139_9d58 GCCCTCATCTCTGCTACCGTTTACATCATCCG(+AGGACA)GTACCTTTGTAGCCTTAGACCGATGATCACC NGG 10 8 17 8 24 0 100 5 10
    240613143138_tdxu AACACGGTGATCATCGGTCTAAGGCTACAAAG(+AGGACA)GTACCGGATGATGTAAACGGTAGCAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240613143137_byg2 CCAACACTTAGAACATTTTTTTAAACAGAAAG(+AGGACA)GTACCAGTACCTAGACACAAACGTTAATGAG NGG 10 8 17 8 24 0 100 5 10