Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143135_eq58 TCGTACTCATTAACGTTTGTGTCTAGGTACTG(+AGGACA)GTACCTTTCTGTTTAAAAAAATGTTCTAAGT NGG 10 8 17 8 24 0 100 5 10
    240613143134_g0es CCAGAGCATATACAAAATTATACAAATTACAG(+AGGACA)GTACCTGGTTTGTAAAACTGACCATCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613143132_939h GAAGTCACCTGATGGTCAGTTTTACAAACCAG(+AGGACA)GTACCTGTAATTTGTATAATTTTGTATATGC NGG 10 8 17 8 24 0 100 5 10
    240613143130_9t0o ATCTACTTGTGGTTCCCCAGACAGCTCTAGGG(+AGGACA)GATCCGAACTGACTCTGTCCATGCATTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613143129_dlay ACCCCTGAGAATGCATGGACAGAGTCAGTTCG(+AGGACA)GATCCCCTAGAGCTGTCTGGGGAACCACAAG NGG 10 8 17 8 24 0 100 5 10
    240613143127_uars CTTCGGGGGAAAGACCCACCCTAACTGTGGTG(+AGGACA)GTACCGTTTCATAGGCTGGGGTCCCAGACTA NGG 10 8 17 8 24 0 100 5 10
    240613143126_gzoy TTATCTAGTCTGGGACCCCAGCCTATGAAACG(+AGGACA)GTACCACCACAGTTAGGGTGGGTCTTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613143125_l884 GTTAGGAGAGGTTAGGAAACCTCTAGAAAGTG(+AGGACA)GATCCTTGCTGGGGGTAATTATATCACTGGG NGG 10 8 17 8 24 0 100 5 10
    240613143124_hv4t CCTACCCCAGTGATATAATTACCCCCAGCAAG(+AGGACA)GATCCACTTTCTAGAGGTTTCCTAACCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613143122_6han GTTTTCACTAGGTATAGAGCAGTAGTCACCAG(+AGGACA)GTACCAACACAGGGAGCCCTCCACACACTCT NGG 10 8 17 8 24 0 100 5 10