Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143349_iywf AGGTGGCTTTCGGGCTACCATGCTCCCTCATG(+AGGACA)GATCCAGCTCTCCCACCCTGTCACCTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613143348_y2qm CTCTCTCCAGTTAGTACCTATCTGGGATATTG(+AGGACA)GTACCTTTGGATATGAATGTCCTTATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613143346_ss9o ATATTTTCTTATAAGGACATTCATATCCAAAG(+AGGACA)GTACCAATATCCCAGATAGGTACTAACTGGA NGG 10 8 17 8 24 0 100 5 10
    240613143345_hpve TGAAACTGGACTTGCTGGGGTGTCGCTAAGAG(+AGGACA)GATCCCATTTGACCTCTGCCTTGCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240613143343_3qgr TCTCAGGCTGTGGCAAGGCAGAGGTCAAATGG(+AGGACA)GATCCTCTTAGCGACACCCCAGCAAGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613143342_5pg8 CTTTGCTGCCTAAGAACCGGAGCCAACAGCAG(+AGGACA)GTACCTCCTGGGGTTCCCTGAGGAGTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613143341_r5zi GTTTTAGCACACTCCTCAGGGAACCCCAGGAG(+AGGACA)GTACCTGCTGTTGGCTCCGGTTCTTAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613143339_z9sh GTAGGGTCCCAAAGGAGAGCCGGGGCAGGGTG(+AGGACA)GATCCCTGCTTTGCTGCCTAAGAACCGGAGC NGG 10 8 17 8 24 0 100 5 10
    240613143337_91ku TGTTGGCTCCGGTTCTTAGGCAGCAAAGCAGG(+AGGACA)GATCCACCCTGCCCCGGCTCTCCTTTGGGAC NGG 10 8 17 8 24 0 100 5 10
    240613143335_tx3p GGCAGAGGGACCTATGGCCAGATGCCAGGGAG(+AGGACA)GTACCCAGAGACCCCACAGCGTGGTTCATGC NGG 10 8 17 8 24 0 100 5 10