Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613143610_ew4p GTCATATATCGTAAATATTTCTCTTCTCAAAG(+AGGACA)GATCCAATAACCAGTTAAGTCCAAGCAATAG NGG 10 8 17 8 24 0 100 5 10
    240613143609_xq8n TTAAGTACTTCATAACCTATTAAGGAAAACAG(+AGGACA)GTACCTGGTGTCTCAGGAAGGCAATCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613143607_073p GTGTGATCTTGATTGCCTTCCTGAGACACCAG(+AGGACA)GTACCTGTTTTCCTTAATAGGTTATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613143604_bg9g CTGGAAGGAGTTAGCTCCCAGAGCTCCCCAGG(+AGGACA)GTACCCTCCCGGGGTCGGCCTTACCTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613143602_cgay TGGAGATGGAAGGTAAGGCCGACCCCGGGAGG(+AGGACA)GTACCCTGGGGAGCTCTGGGAGCTAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240613143600_02i7 TTGAGTTATGGATCGTTAGATCTTCATCCTGG(+AGGACA)GATCCAGTTCCATTTTCTCTTCCGTCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613143558_1m5j TATCCTCAGGGACGGAAGAGAAAATGGAACTG(+AGGACA)GATCCCAGGATGAAGATCTAACGATCCATAA NGG 10 8 17 8 24 0 100 5 10
    240613143557_bs1p GACAGTCCATCCATCCTTAACTGCAGAATCTG(+AGGACA)GATCCCCTGCCCTCCAGTTTTGCTGCCGTTT NGG 10 8 17 8 24 0 100 5 10
    240613143555_0irr TTGATAAACGGCAGCAAAACTGGAGGGCAGGG(+AGGACA)GATCCAGATTCTGCAGTTAAGGATGGATGGA NGG 10 8 17 8 24 0 100 5 10
    240613143554_r0o6 ATATGTGTGTGTGTGTGTGTGTGTGTATATAG(+AGGACA)GTACCTACAATCTTTGTGGAATAGATTCTAA NGG 10 8 17 8 24 0 100 5 10